大鼠脊髓楔型半切空洞模型的建立及评估  

Establishment and Evaluation of a Wedged-block-hemisected Injury Model in Rats Spinal Cord

作　　者：赵富生[1] 武庚[1] 武杨[2] 魏淑芳[2] 郝鑫[1] 李月珍[1] 张际绯[1] 

机构地区：[1]牡丹江医学院,牡丹江157011 [2]牡丹江医学院红旗医院,牡丹江157011

出　　处：《实验动物科学》2013年第4期13-17,共5页Laboratory Animal Science

基　　金：国家自然科学基金项目(No.81171467);黑龙江省卫生科技计划项目资助(No.2010-241)

摘　　要：目的建立一种新型大鼠脊髓半切模型,为临床脊髓空洞损伤研究提供适宜的动物模型。方法 25只SD大鼠随机分为脊髓楔型块状半切组(A组,n=10)、假手术组(B组,n=10)和正常对照组(C组,n=5)。术后采用BBB运动功能评分、斜板试验、HE染色和核磁共振(MRI)检测综合评价脊髓半切模型的稳定性。结果术后A组大鼠伤侧后肢BBB运动功能评分及斜板维持最大角度值显著低于B组和C组(P<0.05),而这些指标在B组和C组之间没有明显差异;术后28 d HE和MRI检测显示脊髓损伤区(A组)灰质和白质发生明显的形态学改变。结论通过该方法建立的脊髓半切模型稳定、可靠,适于脊髓空洞性损伤的研究。Objective Establishment a novel hemisected spinal cord injury model with rats to supply model for clinical research.Method 25 SD rats were randomly divided into wedged-block hemisected group （A group,n =10）,sham operation group （B group,n =10） and control group （C group,n =5）.After operation,the stability of the model was comprehensive evaluated by grading the scores of Basso-Beattie-Bresnahan （BBB）,swash plate test,HE staining and MRI assay.Result After operation,the mean scores of BBB in injured hind limb and maximum angle of swash plate in group A were significantly lower than group C and B （P ＜ 0.05）,but these parameters without different between B group and C group; 28 days after operation,HE staining and MRI assay shows a significant morphological changes in spinal cord tissue of group A; Conclusion The results suggested that the rat model established by this method with high quality of stability and reliability which suitable to evaluate the spinal cord injury research.

关 键 词：脊髓楔型半切 动物模型 大鼠 

分 类 号：R-322[医药卫生]