癌基因c-erbB-2及其蛋白产物表达在乳腺癌淋巴结转移诊断及预后评价中的应用价值  

Application value of cancer gene c-erbB-2 and its protein product expression in evaluation of the diagnosis and prognosis of breast cancer with lymph node metastasis

作　　者：叶红[1] 

机构地区：[1]天门市第一人民医院病理科,湖北省天门431700

出　　处：《中国基层医药》2013年第21期3251-3253,共3页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的 探讨癌基因c-erbB-2及其蛋白产物（P185）的表达在乳腺癌淋巴结转移诊断中的应用价值及c-erbB-2表达与肿瘤病理分型、复发的关系.方法 选择112例乳腺疾病患者为研究对象,根据临床诊断不同分为乳腺癌淋巴结转移组、乳腺癌无转移组及乳腺良性病变组.应用免疫组化法、酶联免疫吸附（ELISA）法和电化学发光法分别对所有患者癌基因c-erbB-2及癌胚抗原（CEA）进行检测.比较三组患者P185、CEA指标的差异以及c-erbB-2表达和CEA对乳腺癌淋巴结转移诊断的灵敏度和特异度,同时分析c-erbB-2表达与肿瘤病理分型及复发的关系.结果 乳腺癌淋巴结转移组和乳腺癌无转移组患者P185和CEA指标均明显高于乳腺良性病变组（t=7.093、6.443、9.232、8.508,均P＜0.01）,乳腺癌淋巴结转移组P185明显高于无转移组（t=2.281,P＜0.05）,而两组间CEA差异无统计学意义（t=0.406,P＞0.05）.癌基因c-erbB-2表达对乳腺癌淋巴结转移的灵敏度与CEA相当（x2=0.632,P＞0.05）,但特异度明显高于CEA检测（x2 =11.900,P＜0.01）.c-erbB-2阳性表达率与病理分型无明显关系.癌基因c-erbB-2表达阳性患者,术后复发率明显高于c-erbB-2表达阴性的患者（x2=5.141,P＜0.05）.结论 癌基因c-erbB-2及其蛋白产物的表达在乳腺癌淋巴结转移的诊断中具有良好的灵敏性和特异性,且与乳腺癌术后复发具有明显的相关性,对乳腺癌的诊断和预后评价具有重要参考价值.Objective To discuss the application value of cancer gene c-erbB-2 and its protein products P185 in evaluation of the diagnosis and prognosis of breast cancer with lymph node metastasis,and to explore the relationship between c-erbB-2 expression and tumor pathology classification,relapse.Methods 112 cases with mammary disease were selected as the research subjects.According to different clinical diagnosis,they were divided into breast cancer lymph node metastasis group,breast cancer without metastasis group and mammary gland benign pathological change group.The c-erbB-2 gene and carcinoembryonic antigen（CEA） were detected by immunohistochemical method,enzyme-linked immunosorbent（ELISA） and electrochemical luminescence method.The c-erbB-2 protein（P185）and CEA of the three groups were compared,the sensitivity and specificity of c-erbB-2 expression and CEA in the diagnosis of breast cancer with lymph node metastasis were analyzed.At the same time,the relationship between cerbB-2 expression and tumor pathology classification,relapse was analyzed.Results P185 and CEA index of breast cancer lymph node metastasis group and breast cancer without metastasis group were significantly higher than those of mammary gland benign pathological change group（t =7.093,6.443,9.232,8.508,all P ＜ 0.0 1）,and P185 index of breast cancer lymph node metastasis group was significantly higher than without metastasis group （t =2.281,P ＜0.05）,and there was no significant difference of CEA between the two breast cancer groups （t =0.406,P ＞ 0.05）.The sensitivity of c-erbB-2 expression in dignosis of breast cancer lymph node metastasis had no difference with CEA （x2 =0.632,P ＞ 0.05）,but the specificity was significantly higher than CEA（x2 =11.900,P ＜ 0.01）.The positive expression rate of c-erbB-2 and tumor pathology classification had no obvious relation：Postoperative recurrence rate of c-erbB-2 positive expression patients was significantly higher than the negative expression patients（x2 =5.141,P ＜

关 键 词：癌基因C-ERBB-2 癌胚抗原 乳腺肿瘤 淋巴结转移 

分 类 号：R737.9[医药卫生—肿瘤]