重组人骨形成蛋白成熟肽-4对辐射小鼠骨髓造血损伤的修复作用  

Restoring effect of rhBMP-4m on bone marrow hematopoietic injury induced by irradiation in mice

作　　者：刘绍凡[1] 陈愉[1] 万锐杰 徐敏敏[1] 

机构地区：[1]重庆市中医院骨科,400016

出　　处：《检验医学与临床》2013年第19期2510-2512,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨小鼠受到辐射后,重组人骨形成蛋白成熟肽-4(rhBMP-4m)对辐射引起的骨髓造血损伤的修复作用。方法通过60 Coγ射线照射,建立小鼠骨髓造血损伤模型,经rhBMP-4m连续治疗后,抽血检测小鼠外周血白细胞数,并进行骨髓单个核细胞计数,对脾结节进行计数并通过流式细胞仪检测骨髓单个核细胞中CD34+细胞比例,比较辐射组和实验组之间的差别。结果经rhBMP-4m治疗的小鼠,白细胞数、单个核细胞数、脾结节数和CD34+细胞比例均明显高于辐射组(P<0.05)。结论对于辐射引起的小鼠骨髓造血损伤,rhBMP-4m能够加快骨髓造血系统的重建。Objective To explore the restoring effect of recombinant human bone morphogenetic protein-4m（rhBMP-4m）on bone marrow hematopoietic injury induced by irradiation in mice.Methods Animal models of bone marrow hematopoietic injury were established by radiating with 60 Coγ-ray.After continuous treatment with rhBMP4m,the number of white blood cells（WBC）in peripheral blood,and total and CD34＋mononuclear cells in bone marrow were detected.Results The number of WBC,mononuclear cells and the proportion of CD34＋cells in experiment group were higher than radiation group（P0.05）.Conclusion rhBMP-4mcould accelerate the restoring process of hematopoietic function in mice with bone marrow hematopoietic injury induced by irradiation.

关 键 词：辐射损伤 重组人骨形成蛋白成熟肽-4 CD34+ 

分 类 号：R336[医药卫生—人体生理学]