正畸力作用下大鼠牙周组织中核因子κB的表达  

Effect of orthodontic force on the expression of nuclear factor-kappa B in periodontium of rats

作　　者：陈媛[1] 刘楚峰[2] 陈洁玉[1] 曹阳[1] 

机构地区：[1]中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室,广州510055 [2]广东省口腔医院·南方医科大学附属口腔医院正畸科

出　　处：《中华口腔医学研究杂志（电子版）》2013年第5期18-21,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：国家自然科学基金(81170990);广东省医学科学研究基金(A2011089);广东省部产学研结合项目(2011B090400097)

摘　　要：目的通过观察大鼠正畸牙移动过程中牙周组织内的核因子κB(NF-κB)表达变化,探讨其在正畸牙移动过程中的作用机制。方法将56只8周龄体重(220±25)g雄性SD大鼠根据正畸力加载时间不同(6 h、12 h、24 h、3 d、7 d、14 d、21 d)分为7组,每组8只。随机选择一侧上颌第一磨牙作为实验牙,对侧第一磨牙作为自身对照牙,采用NiTi拉簧建立大鼠正畸牙移动模型,力值为50 g,分别于相应时间点取正畸牙牙周组织,行免疫组织化学染色检测NF-κB的表达。结果NF-κB在对照侧大鼠牙周组织中弱表达;在正畸移动模型中,NF-κB表达量从加力6 h开始上升,至加力3、7 d后达到峰值,之后逐渐下调,至加力21 d仍稍高于对照牙,压力侧表达大于张力侧。结论NF-κB参与正畸力作用下牙周组织早期的炎症反应和后期的组织改建过程,并且可能更多地参与了牙周改建的骨吸收过程,而对于成骨细胞的作用可能具有一定的相关性,但其具体机制有待进一步研究。Objective To explore the mechanism of periodontal remodeling by observing the expression of nuclear factor-kappa B （NF-KB） in the periodontium of rat molars during orthodontic tooth movement. Methods 56 male SD rats, 8 weeks old and （220±25） g in weight were randomly divided into 6 h, 12 h, 24 h, 3 d, 7 d, 14 d, 21 d, and were loaded with 50 gram orthodontic force on one side of the maxillary first molar to detect the expression NF-KB immunohistochemically, the first molar on the other side were used as the nonappliance control. Results A weakly positive expression can be detected in the controlled groups; in the experimental groups, the concentration increased from 6 h, reaching to the peak at the 3rd day and 7th d, and then decreased until the 21st d, at that time, the positive expressions is still higher than that of the controlled side. The expression of the NF-κB in the pressed zone was higher than that that in the tension zone. Conclusions NF-κB may participate in the inflammatory reaction in the early stage and the reconstruction of the periodontal tissue in the late stage during orthodontic tooth movement of the rat molars. NF-κB may also plays a more important role in osteoclasts, while its role in bone formation needs further exploration.

关 键 词：正畸力 SD大鼠 核因子ΚB 免疫组织化学染色 

分 类 号：R783.5[医药卫生—口腔医学]