浙江汉族长寿人群血脂水平及ApoB基因Xba I-RFLR和3'-VNTR多态性的相关性研究  

Serum lipid levels and ApoB gene Xba I and 3'-VNTR polymorphisms in longevous elderly Han population in Zhejiang

作　　者：盛爱萍[1] 周标[2] 施达[3] 沈毅[4] 

机构地区：[1]金华职业技术学院,浙江金华321017 [2]浙江省疾病预防控制中心,浙江杭州310051 [3]金华市疾病预防控制中心,浙江金华321002 [4]浙江大学,浙江杭州310058

出　　处：《中国病理生理杂志》2013年第10期1826-1831,共6页Chinese Journal of Pathophysiology

基　　金：浙江省金华市科技局项目(No.2010-3-075)

摘　　要：目的:研究浙江汉族长寿人群的血脂水平及载脂蛋白B(ApoB)基因Xba I-限制性片段长度多态性(RFLP)和3’-可变数目串联重复序列(VNTR)多态性对血脂四项和载脂蛋白水平是否有影响,了解长寿发生的机制。方法:采用体格检查与实验室研究分别对长寿老人组、长寿子女组、正常对照组和非长寿子女组进行血样采集、ApoB基因Xba I-RFLP和3'-VNTR分型。结果:血清甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)及ApoB与长寿呈负相关(P<0.05),高密度脂蛋白胆固醇(HDL-C)与长寿呈正相关(P<0.01)。拥有X+X-基因型的人,除TG外,TC、LDL-C及ApoB均高于拥有X-X-基因型的人(P<0.01),其HDL-C低于拥有X-X-基因型的人(P<0.01);拥有大等位基因的人,其TC、TG、LDL-C及ApoB均高于拥有小等位基因的人(P<0.01),而HDL-C含量较低(P<0.01)。结论:高TG、LDL-C、ApoB和低HDL-C不利于长寿,拥有X-X-基因型和3'-VNTR分型小等位基因的人血脂水平较健康,也利于长寿。AIM： To study the levels of serum lipids and the effects of apolipoprotein B （ApoB） gene Xba I-restriction fragment length polymorphism （RFLP） and 3＇-variable-number tandem repeat （VNTR） polymorphism on serum lipid and apolipoprotein levels in longevous elderly Han population in Zhejiang. METHODS： Physical and laboratory examinations were performed on longevous elders, children of longevous elders, normal control people and children of non-longevous elders, and the Xba I-RFLP and 3＇-VNTR polymorphism in ApoB gene were genotyped. RESULTS： The serum levels of triglyceride （TG）, low-density lipoprotein cholesterol （LDL-C） and ApoB were negatively correlated with longevity （P〈0.05）, while the level of high-density lipoprotein cholesterol （HDL-C） was positively correlated with longevity （P〈0.01）. The levels of total cholesterol （TC）, LDL-C and ApoB were higher and the HDL-C level was lower in the people with Xba I-RFLP X＋X- genotype than those in the people with Xba I-RFLP X-X- genotype （P〈0.01）. The levels of TC, TG, LDL-C and ApoB were higher and the HDL-C level was lower in the people with 3＇-VNTR large allele than those in the people with 3＇-VNTR minor allele （P〈0.01）. CONCLUSION： High TG, LDL-C and ApoB levels and low HDL-C level are not conductive to longevity. Serum lipid levels in the people with Xba I-RFLP X-X- genotype or 3＇-VNTR minor allele are healthy and conductive to longevity.

关 键 词：长寿 脂质 载脂蛋白B 

分 类 号：R161.7[医药卫生—公共卫生与预防医学]