BTG1在HK-2和786-O细胞株中的表达及其对细胞周期和细胞凋亡的影响  

Expression of BTG1 in HK-2 and 786-O and its influence on cell cycle and apoptosis of HK-2 and 786-O

作　　者：刘春辉[1] 许斌[1,2] 张磊[1] 卢凯[1] 陈恕求[1,2] 陈明[1,2] 

机构地区：[1]东南大学医学院,江苏南京210009 [2]东南大学附属中大医院泌尿外科,江苏南京210009

出　　处：《东南大学学报（医学版）》2013年第5期533-538,共6页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目(81070592;81202034)

摘　　要：目的:探讨B细胞易位基因1(BTG1)在人肾小管上皮细胞株(HK-2)和肾透明细胞腺癌细胞株(786-O)中的表达及BTG1基因对HK-2和786-O细胞周期、细胞凋亡的影响。方法:采用Western blotting方法检测BTG1蛋白在HK-2、786-O细胞株中的表达,构建BTG1表达质粒转染786-O细胞,合成BTG1干扰RNA转染HK-2细胞,通过流式细胞仪(FACS)观察BTG1对HK-2、786-O细胞周期和细胞凋亡的影响。结果:Western blotting检测显示,BTG1蛋白在HK-2中的表达高于786-O。786-O中高表达BTG1蛋白能够促进其凋亡,增加其G0/G1期比率;HK-2中低表达BTG1蛋白能够抑制其凋亡,减少其G0/G1期比率。结论:BTG1可能通过对细胞凋亡及细胞周期的调控抑制肾细胞癌的生长。Objective：To explore the expression of B-cell translocation gene 1 （ BTG1 ） in normal renal tubule epithelial cell lines （HK-2） and renal clear cell cancer cell lines （786-O） and its influence on cell cycle and apoptosis of HK-2 and 786-O.Methods： The expression of BTG1 in HK-2 and 786-O was detected by Western blotting.The BTG1 expression plasmid was constructed and transfected into 786-O.The interference RNA of BTG1 was designed and transfected into HK-2.The effect of BTG1 on cell cycle and apoptosis of HK-2 and 786-O was observed by flow cytometry （FACS）.Results：The expression of BTG1 protein was higher in HK-2 than in 786-O. High expression of BTG 1 would promote cell apoptosis and increase the rate of G 0/G1 phase in 786-O.Low expression of BTG1 would inhibit the cell apoptosis and reduce rate of G 0/G1 phase in HK-2.Conclusion：BTG1 can inhibit the growth of renal cell carcinoma through regulation of cell cycle and apoptosis .

关 键 词：肾透明细胞癌 细胞易位基因 细胞周期 细胞凋亡 

分 类 号：R737.14[医药卫生—肿瘤] Q782[医药卫生—临床医学]