健脾复肝丸质量标准研究  

Study on Quality Standard of Jianpi Fugan Pills

作　　者：郑光明 宋宏安 

机构地区：[1]荆门市中医医院,湖北荆门448000

出　　处：《中国中医药信息杂志》2013年第11期47-49,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立健脾复肝丸的质量标准。方法采用薄层色谱法对当归、白术进行定性鉴别。采用高效液相色谱法测定阿魏酸含量。色谱柱:TIANHE C18(250 mm×4.6 mm,5μm);流动相:乙腈-0.085%磷酸(17∶83);流速:1.0 mL/min;柱温:25℃;检测波长:323 nm。结果健脾复肝丸中当归、白术的供试品色谱中,在与对照品色谱相应位置上显相同颜色斑点。阿魏酸对照品在0.114～1.482μg范围内线性关系良好,平均加样回收率为98.36%,RSD=1.16%。结论本方法简便准确、灵敏度高,可用于健脾复肝丸的质量控制。Objective To establish the quality standard for Jianpi Fugan Pills. Methods Angelicae Sinenis Radix and Atractylodis Macrocephalae Rhizome in the formulation were qualitatively identified by TLC. The content of ferulic acid was analyzed by HPLC. TIANHE C18 column （250 min×4.6 mm, 5μm） was used with a mobile phase of acetonitrile-0.085% phosphorous acid （17 ： 83）. The content was detected at wavelength of 323 nm. The flow rate was 1.0 mL/min, and temperature was 25℃. Results The spots in the TLC of Angelicae Sinenis Radix and Atractylodis Macrocephalae Rhizome were in the same chormatograms of control articles. The linear range of ferulic acid was 0.114-1.482μg. The average recovery was 98.36% and RSD was 1.16%. Conclusion The method is simple, accurate and highly sensitive, which can be used for quality control of Jianpi Fugan Pills.

关 键 词：健脾复肝丸 阿魏酸 薄层色谱法 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]