福建正红菇遗传多样性分析  

Phylogenetic Diversity of Russula griseocarnosa from Fujian

作　　者：肖冬来[1] 陈宇航[1] 杨菁[1] 黄小菁[1] 

机构地区：[1]福建省农业科学院食用菌研究所,福建福州350003

出　　处：《福建农业学报》2013年第9期902-905,共4页Fujian Journal of Agricultural Sciences

基　　金：福建省自然科学基金项目(2011J05056);福建省农业科学院青年科技人才创新基金项目(2010BS-1)

摘　　要：以采自福建省山区的正红菇Russula griseocarnosa为研究材料,进行ITS(internal transcribed spacer)、RPB2(the second largest subunit of the nuclear RNA polymerase enzyme II)保守区段克隆测序和序列同源性比对。将从GenBank中获得的部分红菇属物种ITS和RPB2序列与正红菇序列通过最大简约法进行系统发育分析。基于ITS序列构建的系统发育树表明,福建正红菇与云南大红菌Russula griseocarnosa X.H.Wang,Zhu L.Yang&Knudsen,sp.nov.间序列差异较小,亲缘关系较近,以99%的支持率聚为一簇。福建正红菇与欧洲红菇Russula vinosa及玫瑰红菇Russula rosea间序列差异较大,亲缘关系较远,在系统发育树上聚为不同分枝。基于RPB2序列构建的系统发育树也表明福建正红菇与云南大红菌聚为一簇(支持率94%),而与玫瑰红菇聚为不同分支。Fruiting bodies of Russula griseocarnosa were collected in Fujian to study the mushroom′s phylogenetic diversity .Two gene regions of ITS （ internal transcribed spacer） and RPB2（the second largest subunit of the nuclear RNA polymerase enzyme II ） were cloned .The phylogenetic diversity was examined according to the maximum parsimony trees with sequences of several other Russula species .It showed that ,based on the ITS sequences ,R . griseocarnosa found in the province clustered with R .griseocarnosa X .H .Wang ,Zhu L .Yang ＆ Knudsen ,sp .nov . found in Yunnan ,with a bootstrap value of 99% .The differences among R .griseocarnosa ,R .vinosa and R .rosea were apparent ,with a bootstrap value below 50% .The maximum parsimony tree based on the RPB2 sequences also showed that R .griseocarnosa clustered with R .griseocarnosa X .H .Wang , Zhu L .Yang ＆ Knudsen , with a bootstrap value of 94% .The distinction was also apparent ,with a bootstrap value less than 50% .

关 键 词：正红菇 菌根菌 遗传多样性 ITS RPB2 

分 类 号：S646.9[农业科学—蔬菜学]