藻胆蛋白的提取、纯化及其体外抗紫外活性  

Extraction and purification of phycobiliprotein and its anti-ultraviolet activity in vitro

作　　者：叶翠芳[1] 杨珂[1] 张美英[1] 汤顺清[2] 王一飞[1] 利奕成[1] 

机构地区：[1]暨南大学生物医药研究开发基地,广东广州510632 [2]暨南大学生物医学工程系,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2013年第5期522-526,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：十二五国家科技支撑计划项目(SQ2011SF12B02099);广州市天河区科技计划项目(104ZH134)

摘　　要：本研究采用饱和硫酸铵盐析沉淀法和DEAE-纤维素离子交换层析法提取并纯化藻胆蛋白,通过体外细胞实验,建立小鼠NIH 3T3成纤维细胞紫外损伤模型,测定藻胆蛋白对小鼠NIH 3T3细胞生物活性和抗紫外辐射活性.实验结果显示,从紫菜中可分离纯化得到纯度达到3.0以上的藻胆蛋白,藻胆蛋白对小鼠NIH 3T3成纤维细胞具有显著的促增殖作用,并对UVA辐射损伤的小鼠NIH 3T3成纤维细胞具有修复作用.本研究结果将对藻胆蛋白在护肤领域的应用提供理论支持.Study on extraction and purification of phycobiliprotein from laver by saturated solid ammo- nium sulphated deposition and DEAE-cellulose chromatography. Test biological activity and anti-ultravio- let capability of purified phycobiliprotein, though vitro cells experiment of NIH 3T3 mouse fibroblasts ex- posed to UVA. From testing results, phycobiliprotein of purified ratio above 3.0 can be obtained after ex- acting and treating from laver. The purified phycobiliprotein has good accelerated proliferation effect on NIH 3T3 mouse fibroblasts and good repairing effect when exposed to UVA. This research will work for skin care cosmetic of phycobiliprotein as theory support.

关 键 词：藻胆蛋白 提取 纯化 抗紫外辐射 小鼠NIH 3T3成纤维细胞 

分 类 号：Q-331[生物学] TS974.1[轻工技术与工程]