微流控芯片测定猪肝中的盐酸克伦特罗  

Determination of Clenbuterol in Pig Liver by Microfluidic Chip

作　　者：张兰春[1] 苏子豪[1] 翟海云[1] 周清[1] 潘育方[1] 

机构地区：[1]广东药学院,广东广州510006

出　　处：《宜春学院学报》2013年第9期9-11,共3页Journal of Yichun University

基　　金：国家自然科学基金项目(21005021);广东省科技计划项目(2010B020316010)

摘　　要：建立了微流控芯片非接触电导检测法测定猪肝中盐酸克伦特罗的含量.优化选择5.0mmol/L HAc为缓冲溶液,进样时间为15s,分离电压为2.0kV,盐酸克伦特罗0.4min内可得到很好的分离.线性范围为5.0～60.0μg/mL（r=0.998）,检出限为2.0 μg/mL （S/N =2）,相对标准偏差（RSD）为1.1％,平均加标回收率为94.4％.The clenbuterol in pig liver was determined by microfluidie chip with contaetless conductivity detection with 5.0 mmol/L HAe as buffer solution. The elenbuterol was separated and detected in 0. 4 min at the separation voltage of 2. 0 kV and injection time 15s. Clenbuterol showed good linear response in the range of 5.0 - 60.0 μg/mL （r =0. 998） , with the limit of detection was 2. 0 μg/ mL （S/N =2）, and RSD was 1.1% （n=6） . The average recoveries was 94.4% （RSD =1.7%, n=6） .

关 键 词：微流控芯片 非接触电导检测 猪肝 盐酸克伦特罗 

分 类 号：O657.63[理学—分析化学]