九株蛹虫草菌株的生物学性状研究  

Study on the Biological Characteristics of Nine Cordyceps militaris Strains

作　　者：刘洋[1] 孙来玉[1] 陆云华[1] 韩志萍[1] 

机构地区：[1]湖州师范学院生命科学院,浙江湖州313000

出　　处：《北方园艺》2013年第20期140-143,共4页Northern Horticulture

基　　金：湖州市自然科学资金资助项目(2010C50128);浙江省自然科学基金资助项目(Y5110067)

摘　　要：比较了9种蛹虫草菌株的生物学性状,并对菌株的ITS序列,菌丝体及发酵液的虫草素含量进行了测定。结果表明:菌株‘GIM 5.502’、‘GIM 5.512’、‘GIM 5.270’、‘GIM 5.272’、‘GIM 5.266’5个菌株可以正常转色,其余4个菌株不能正常转色,这9个菌株均未能正常发育形成子实体。9个蛹虫草菌株的ITS序列分析表明,ITS序列变异极小。菌株‘GIM 5.502’的菌丝体和发酵液中所含的虫草素最高,分别为6.971、8.461mg/L,而菌株‘GIM 5.267’、‘GIM 5.272’的菌丝体及发酵液中,未检测到虫草素。The biological characteristics of nine Cordyceps militaris strains were studied. The ITS sequence of the nine strains were analyzed and the cordycepin concentration in the mycelium and fermentation broth were also determined. The results showed that strain ＇GIM 5. 502＇, ＇GIM 5. 512＇, ＇GIM 5. 270＇, ＇GIM 5. 272＇, ＇GIM 5. 266 ＇could change color in mycelium normally,but they could not develop fruit body regularly,the other four strains could neither change color in mycelium normally,nor form fruit body. The ITS sequence analysis showed that it had little change between different Cordyceps militaris strains. The cordyeepin concentration in the mycelium and fermentation broth of the strain＇ GIM 5. 502＇ were the highest among that of the other strains, which was up to 6. 971 mg/L, 8. 461 mg/L respectively. However,the cordycepin could not be assayed in that of strain＇GIM 5. 267＇,＇GIM 5. 272＇

关 键 词：蛹虫草 生物学性状 ITS 虫草素 

分 类 号：S567[农业科学—中草药栽培]