毒三素链霉菌SIPI-2012406抑菌活性产物的分离纯化和结构鉴定  

Isolation, Purification and Structural Identification of Antimicrobial Products from Streptomyces toxytricini SIPI-2012406

作　　者：胡莎莎[1] 吴勇[1] 顾鸽青[1] 张喜全[2] 冯军[1] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院,创新药物与制药工艺国家重点实验室,上海200040 [2]江苏正大天晴药业股份有限公司,江苏南京210038

出　　处：《中国医药工业杂志》2013年第10期980-985,共6页Chinese Journal of Pharmaceuticals

基　　金：国家"重大新药创制"科技重大专项(2012ZX9301002003-004)

摘　　要：利用大孔吸附、阳离子交换和葡聚糖凝胶过滤等色谱方法从毒三素链霉菌SIPI-2012406发酵液中分离纯化得到3个活性化合物,纯度均达99.5%。抗菌活性测定结果显示,其对枯草芽孢杆菌和金黄色葡萄球菌的MIC均不超过0.05 g/ml。其中两个化合物的紫外光谱、红外光谱、高分辨质谱和核磁共振谱等研究表明,它们为链丝菌素C和链丝菌素B。该结果说明毒三素链霉菌能够产生链丝菌素类化合物。Three antimicrobial products were obtained by chromatographic purification on macroporous resin, cation exchange resin and Sephadex LH-20 columns from fermentation broths of Streptomyces toxytricini SIPI-2012406. All of their purities were 99.5 %. The results of antimicrobial activity assays showed that their MIC to B. subtilis and S. aureus were not exceeding 0.05 μg/ml. The studies of UV, IR, HRMS and NMR of two compounds indicated that they were streptothricin C and streptothricin B. The results showed that Streptomyces toxytricini could produce streptothricin compounds.

关 键 词：毒三素链霉菌 链丝菌素 分离 纯化 

分 类 号：TQ465[化学工程—制药化工]