Dysadherin、E-cadherin在宫颈癌中的表达及意义  

The expressions of dysadherin and E-cadherin and their significance in cervical caner

作　　者：吴艳[1] 傅芬[2] 周艳芳[3] 

机构地区：[1]赣南医学院第一附属医院妇产科,江西省赣州市341000 [2]南昌大学第二附属医院妇产科,330006 [3]江西省肿瘤医院妇科,南昌市330006

出　　处：《实用医学杂志》2013年第20期3317-3319,共3页The Journal of Practical Medicine

基　　金：江西省教育厅2013年科学技术研究项目(编号:GJJ13156)

摘　　要：目的:探讨宫颈癌中dysadherin、E-cadherin的表达及意义。方法:免疫组化及RT-PCR法检测宫颈癌组织中两者蛋白和mRNA表达水平。结果:宫颈癌组dysadherin蛋白表达高于正常组,E-cad表达低于正常组(P<0.05)。dysadherin蛋白高表达及E-cad蛋白低表达与宫颈癌病理分级、临床分期及淋巴结转移相关(P<0.05),而与年龄无关(P>0.05)。宫颈癌组dysadherin mRNA表达(1.05±0.14)高于正常组(0.91±0.09),E-cad mRNA表达(0.64±0.07)低于正常组(0.89±0.11)。dysadherin与E-cad表达呈负相关。结论:联合dysadherin、E-cad检测可作为宫颈癌诊断的辅助指标。Objective To investigate the expressions of dysadherin and E-cadherin and their clinical significance in cervical cancer. Methods The mRNA and protein expressions of dysadherin and E-cadherin in cervical cancer tissues were detected by immunohistochemistry and RT-PCR, respectively. Results The expression of dysadherin protein was significantly higher and that of E-cadherin protein was significantly lower in cervical cancer than those in normal cervical tissues, respectively （P 〈 0.05）. High expression of dysadherin and low expression of E-cadherin were related to the pathological grade, clinical stage, and the presence of lymph node metastasis of cervical cancer （P 〈 0.05 ）, but had nothing to do with age （P 〉 0.05 ）. The expression of dysadherin mRNA （1.05 ± 0.14） was higher and that of E-cadherin mRNA （0.64 ± 0.07） was lower in cervical cancer than those in normal cervical tissues （0.91 ± 0.09 and 0.89 ± 0.11 ）, respectively. There was negative correlation between the expression of dysadherin and E-cad. Conclusions The combined detection of dysadherin and E-eadherin could be used as a potential marker for the diagnosis of cervical cancer.

关 键 词：宫颈肿瘤 DYSADHERIN E-CADHERIN 免疫组化 RT-PCR 

分 类 号：R737.33[医药卫生—肿瘤]