快速膜乳化法制备载醋酸曲普瑞林PLGA微球  

Preparation of Triptorelin Acetate-loaded PLGA Microspheres by Premix Membrane Emulsification

作　　者：王宁[1,2] 王玉霞[2] 秦培勇[1] 韦祎[2] 马光辉[2] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190

出　　处：《过程工程学报》2013年第5期862-869,共8页The Chinese Journal of Process Engineering

基　　金：国家自然科学基金资助项目(编号:51173187;20706053)

摘　　要：以生物可降解聚合物聚(乳酸羟基乙酸)(PLGA)为载体,以160 g/L明胶水溶液为内水相、含500 g/L PLGA的二氯甲烷为油相,采用快速膜乳化和溶剂蒸发法制备了粒径均一的载醋酸曲普瑞林PLGA微球,微球粒径约30 m,粒径分布系数Span<0.8,醋酸曲普瑞林包埋率达80.12%,药物在磷酸盐缓冲液中释放36 d的释放率为72.60%,体外释放行为良好.Triptorelin acetate loaded biodegradable poly（lactic-co-glycolic acid） （PLGA） uniform-sized microspheres were prepared by a method combining premix membrane emulsification and solvent evaporation with high concentration （160 g/L） gelatin as water phase and methylene chloride containing high concentration （500 g/L） PLGA as oil phase. It was possible for the drug completely entrapped by the PLGA microcapsules. The mean diameter ofPLGA microspheres was about 30 μm, the encapsulation efficiency of triptorelin acetate 80.12%, and Span （coefficient of size distribution） value less than 0.8. Subsequently, the cumulative release rate of PLGA microspheres loading triptorelin in vitro for 36 d was 72.60%. Furthermore, the release profile of the drug in vitro was well for controlled release.

关 键 词：醋酸曲普瑞林 聚(乳酸-羟基乙酸) 微球 快速膜乳化 包埋率 药物释放 

分 类 号：R944.5[医药卫生—药剂学]