PDLIM4基因低表达与急性髓系白血病患者预后良好相关(英文)  

Reduced Expression of PDLIM4 Gene Correlates with Good Prognosis in Acute Myeloid Leukemia

作　　者：李云[1] 钱军[1] 林江[2] 钱炜[2] 杨静[1] 柴海彦[1] 王翠竹[1] 邓兆群[2] 姚冬明[1] 陈芹[2] 马吉春[2] 

机构地区：[1]江苏大学附属人民医院血液科,江苏镇江212002 [2]江苏大学附属人民医院中心实验室,江苏镇江212002

出　　处：《中国实验血液学杂志》2013年第5期1111-1115,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目(编号81270630,81172592);江苏省科技厅临床医学科技专项课题(编号BL2012056);江苏省"333工程"资助项目(编号BRA2011085);镇江市社会发展资助项目(编号SH2010015,SH2011056)

摘　　要：本实验旨在研究急性髓系白血病(AML)患者中PDLIM4基因表达模型,并分析其临床相关性。运用EvaGreen实时定量PCR(RQ-PCR)方法检测AML患者中PDLIM4基因表达量。结果表明,与21例对照相比,94例AML患者PDLIM4转录本水平降低非常显著(P<0.01),共有42例(45%)AML患者为PDLIM4转录本低表达,M1/M2/M3亚型的PDLIM4低表达率非常显著地高于M4/M5/M6亚型(56%对20%,P<0.01),AML中PDLIM4低表达患者的总体生存时间(OS)显著长于非低表达者(P<0.05);受试者工作特征曲线(ROC)分析显示,ROC曲线下面积为0.865(95%可信区间:0.801-0.930),表明PDLIM4低表达对AML具有诊断意义。结论:PDLIM4低表达是一个常见事件,且对AML预后具有良性影响,可能成为潜在的诊断肿瘤的分子标记。This study was aimed to investigate the expression pattern of gene PDLIM4 （PDZ and LIM domain 4） and analyze its clinical correlation with the patients suffered from acute myeloid leukemia （AML）. The expression pattern of PDLIM4 in AML was detected by using EvaGreen real-time quantitative PCR （ RQ-PCR）. The results showed that the PDLIM4 transcript significantly decreased in 94 AML patients, compared with 21 controls （ P 〈 0.01 ）. The decrease of PDLIM4 transcript was found in 42 （45%） AML patients. PDLIM4 low-rexpression occurred among the subtypes of M1/M2/M3 more frequently than that in M4/M5/M6 （56% vs 20%, P 〈 0.01 ）. AML patients with PDLIM4 low-expression had an overall survival （OS） higher than that in AML patients without PDLIM4 low-expression （P 〈0.05）. Analysis with receiver operating characteristic curve （ROC） displayed that PDL1M4 expression possesses the diagnostic value to differentiate AML from controls, with ROC curve area of 0. 865 （95% CI： 0. 801 -0. 930）. It is concluded that reduced PDLIM4 expression is a common and favorable event for the good prognosis in AML, and can be used as a potential diagnostic biomarker of cancer.

关 键 词：PDLIM4 实时定量PCR 急性髓系白血病 预后 

分 类 号：R733.71[医药卫生—肿瘤]