大鼠“涌泉”穴区相关神经元及其纤维投射的节段和区域性分布——霍乱毒素亚单位B法  

Segmental and Regional Distribution of Neurons and Their Axonal Projection Associated with Acupoint “Yongquan” (KI 1) in the Rat:Cholera Toxin Subunit B Method

作　　者：哈丽娟[1,2] 崔晶晶[1] 朱新龙[1] 王富春[2] 景向红[1] 白万柱[1] 

机构地区：[1]中国中医科学院针灸研究所,北京100700 [2]长春中医药大学,长春130117

出　　处：《针刺研究》2013年第5期375-379,共5页Acupuncture Research

基　　金：国家自然科学基金资助项目(No.81072759);国家重点基础研究发展计划("973"计划)资助项目(No.2010CB 530507;No 2011CB 505201)

摘　　要：目的:用霍乱毒素亚单位B(CTB)神经示踪技术揭示涌泉穴区相关神经元和神经纤维在神经系统中的分布特征。方法:将5μL 1%CTB分别注入5只SD大鼠后足掌心相当于足少阴肾经的"涌泉"穴的部位。3d后将实验动物灌流固定,取出脊神经节、脊髓和脑。然后将各部位组织进行切片并用免疫荧光和免疫组织化学染色技术显示CTB标记的神经成分。结果:被CTB标记的神经成分均出现在示踪剂注入侧的相关神经组织,包括感觉和运动神经元以及跨神经节纤维投射。其中感觉神经元位于腰3至腰5的脊神经节中,以腰4居多;运动神经元位于腰3至腰6脊髓前角的后外侧部,以腰5居多;跨神经节纤维投射分布在腰3至腰5脊髓后角第3、4层的内侧端,并上达至薄束核。结论:应用CTB神经示踪技术有效地揭示了"涌泉"穴区的神经支配,其相关神经元及其纤维投射呈节段或区域性分布,分别位于脊神经节、脊髓前后角和薄束核。Objective To observe the distribution of neurons and （KI 1） area by neural tracing technique with cholera toxin subunit B （CTB） their axonal projection associated with ＂Yongquan＂ Methods A total of 5 L of 1% CTB solution was injected into the front central part of hind foot plantar （corresponding to the KI 1 region in the human body） using a Hamilton mi crosyringe. After 3 survival days, the rats were deeply anesthetized and transcardically perfused for collecting the dorsal root gan-glia （DRGs）, spinal cord and brain tissues. Following fixing in 4% paraforldehyde containing phosphate buffer solution （PBS） and incubation in PBS containing 25% glucose solution, the aforementioned tissues were sectioned to be stained with immunofluores cence or immunohistochemistry for revealing the labeled sensory neurons and their tansgangiionic projection and motor neurons. Results All the labeled neurons （sensory and motor neurons） and transganglionic axonal projection appeared ipsilaterally to the injection side. The labeled sensory neurons were located in the DRGs of lumbar 3-5 segments （L 3-L 5） with a higher concen- tration at L 4, while motor neurons distributed in the dorsolateral portion of spinal ventral horn from L 3 to L 6 with a higher con centration at L 5. In addition, transganglionic axonal projections were found to situate in the medial part of laminae Ⅲ - lV from L 3 to L 5, as far as in the gracile nucleus. Conclusion Acupoint KI 1 area is innervated by sensory neurons in L 3-L 5 DRGs and motor neurons from the dorsolateral ventral horns of L 3 - L 6. The axonal projection of the primary sensory neurons distributes in the medial part of laminae Ⅲ-IV of L 3-L 5 and gracile nucleus, respectively.

关 键 词：涌泉 神经支配 神经示踪 霍乱毒素亚单位B 感觉神经元 运动神经元 

分 类 号：R224.2[医药卫生—中医基础理论]