RAS蛋白激酶类似物-1和细胞外信号调节激酶在胃癌中的表达及其临床意义  

Clinical significance of expression of Ras protein activator like 1 and extracellular regulated protein kinase in gastric cancer

作　　者：赵艳春[1,2] 陈洪[3] 孙林[4] 邱梅 陈旭东 

机构地区：[1]东南大学医学院,江苏省南京市210009 [2]盐城市第二人民医院消化科,江苏省盐城市224003 [3]东南大学附属中大医院消化科,江苏省南京市210009 [4]盐城市第二人民医院病理科,江苏省盐城市224003

出　　处：《世界华人消化杂志》2013年第28期2929-2936,共8页World Chinese Journal of Digestology

基　　金：江苏省自然科学基金资助项目;No.BK2008301~~

摘　　要：目的:研究观察胃癌及癌旁组织中Ras蛋白激酶类似物-1(Ras protein activator like 1,RASAL1)和细胞外信号调节激酶(extracellular regulated protein kinases,ERK)的表达情况,并分析其与胃癌临床病理特征的关系.方法:采用免疫组织化学方法检测60例胃癌组织及其对应癌旁组织中RASAL1、ERK以及磷酸化ERK(p-ERK)的表达水平,并结合胃癌临床病理资料进行分析.结果:RASAL1在胃癌组织中呈弱表达或者无表达,而在癌旁组织中呈强阳性表达,两组间表达差异有统计学意义(秩均数分别为35.84和85.16,P<0.001).ERK在胃癌和癌旁组织中表达无明显差异(秩均数分别为60.68和60.33,P>0.05).p-ERK在胃癌组织中的水平明显高于癌旁组织中的水平,两组间表达差异有统计学意义(秩均数分别为84.93和36.07,P<0.001),且RASAL1的表达下降、p-ERK的过度表达与肿瘤大小、分期、浸润深度、分化程度及淋巴结转移明显相关(均P<0.05).结论:RASAL1低表达和p-ERK过度表达可能与胃癌的发生、发展有关,而RASAL1可能通过调控ERK的活化,使之成为p-ERK,促进胃癌发生及进展.AIM： To investigate the expression of Ras pro-tein activator like 1 （RASAL1） and extracellular regulated protein kinase （ERK） in gastric cancer and to analyze their association with clinico- pathological features of gastric cancer. METHODS： The expression of RASAL1, ERK and phosphorylated ERK （p-ERK） was detected by immunohistochemistry in 60 gastric cancer tissue specimens and matched tumor-adjacent tissue specimens. The association of RASAL1 and ERK expression with clinicopathologic char- acteristics of gastric cancer was then analyzed. RESULTS： RASAL1 expression was weak or un- detectable in gastric cancer but strong in tumor- adjacent tissue （mean rank score： 35.84 vs 85.16, P 〈 0.01）. No significant difference was observed in ERK expression between gastric cancer and tumor-adjacent tissue （mean rank score： 60.68 vs 60.33, P 〉 0.05）. The expression level of p-ERK in gastric cancer was significantly higher than that in tumor-adjacent tissue （mean rank score： 84.93 vs 36.07, P 〈 0.0001）. Besides, decreased ex- pression of RASAL1 and over-activation of ERK were significantly correlated with tumor size, stage, depth of invasion, grade and lymph node metastasis （all P 〈 0.05）. CONCLUSION： Low expression of RASAL1 and high expression of p-ERK may be involved in the development and progression of gastric can- cer. RASAL1 promotes gastric cancer occurrence and development possibly by regulating the ac- tivation of ERK.

关 键 词：胃癌 Ras蛋白激酶类似物-1 细胞外信号调节激酶 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]