Hippo通路中Yes相关蛋白在胰腺癌中的表达及意义  

Significance of expression of Yes-associated protein in pancreatic cancer

作　　者：李晓宇[1] 田字彬[1] 单信芝[1] 张翠萍[1] 刘晓英[1] 徐永红[1] 李新华[1] 武军[1] 赵文君[1] 李丹[1] 

机构地区：[1]青岛大学医学院附属医院消化科,山东省青岛市266003

出　　处：《世界华人消化杂志》2013年第28期3013-3018,共6页World Chinese Journal of Digestology

摘　　要：目的:检测Hippo-YAP信号通路中主要效应蛋白Yes相关蛋白(Yes-associated protein,YAP)在正常胰腺组织和胰腺癌组织中的表达,探讨其表达强度与临床病理因素之间的关系及意义.方法:应用免疫组织化学EnVision法测定Hippo信号通路中YAP蛋白在45例胰腺癌及15例正常胰腺组织中的表达,应用RT-PCR方法检测YAP mRNA在45例胰腺癌及15例正常胰腺组织中的表达.结果:胰腺癌组织中YAP蛋白主要表达于胰腺导管上皮细胞,45例胰腺癌组织中YAP的阳性表达率为93.33%(42/45),15例正常胰腺组织中YAP阳性表达率为26.67%(4/15),两组间的表达差异具有统计学意义(2=27.95,P<0.01).在胰腺癌组织中YAP的表达与肿瘤分化程度密切相关(P=0.048),与年龄、性别、吸烟、饮酒、肥胖、糖耐量异常、糖尿病、慢性胰腺炎及临床分期等无关(P值分别是0.577、0.565、0.541、0.224、1.000、0.542、0.555、0.571、0.926).胰腺癌患者血清CA19-9水平与胰腺癌组织中YAP阳性程度呈正相关.YAP m R N A在胰腺癌组织中的表达较正常胰腺组织明显升高(0.3682±0.0783 vs 0.0394±0.0091,P<0.01).结论:Hippo-YAP通路可能在胰腺癌的发生、发展中发挥重要作用.并有可能成为胰腺癌治疗中新的靶点.AIM： To detect the expression of Yes-associated protein（YAP）,a component of the Hippo signaling pathway,in human pancreatic cancer,and to explore the correlation between the expression of YAP and clinicopathologic features of pancreatic cancer.METHODS： Immunohistochemistry was used to investigate the protein expression of YAP in 45pancreatic cancer specimens and 15 normal pancreatic tissue specimens.The mRNA expression of YAP was detected by RT-PCR.RESULTS： YAP was mainly expressed in pancreatic ductal epithelial cells.The positive rate of YAPexpression was significantly higher in pancreatic cancer than in normal pancreatic tissue（93.33% vs26.67%,P 0.05）.The mRNA expression of YAP in pancreatic cancer was significantly higher than that in normal pancreatic tissue（0.3682 ± 0.0783 vs0.0394 ± 0.0091,P 0.05）.The expression of YAP was associated with degree of differentiation of pancreatic cancer but had no correlation with age,lymph node metastasis,clinical stage,smoking,drinking,or diabetes mellitus.CONCLUSION： The expression of YAP may play an important role in the development of pancreatic cancer,and YAP may be a new target for therapy of pancreatic cancer

关 键 词：Yes相关蛋白 Hippo信号通路 胰腺癌 导管上皮细胞 

分 类 号：R735.9[医药卫生—肿瘤]