糖基转移酶编码基因snogE中断对诺加霉素生物合成的影响  

Effect of gene disruption of snogE encoding glycosyltransferase on nogalamycin biosynthesis

作　　者：石煊雯[1,2] 李梦茜[1,2] 李航 李继安[2] 陈代杰[2] 邵雷[2] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]中国医药工业研究总院上海医药工业研究院创新药物与制药工艺国家重点实验室,上海200040 [3]雅本化学股份有限公司,苏州215433

出　　处：《中国药科大学学报》2013年第5期460-464,共5页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.81172962);国家"重大新药创制"科技重大专项资助项目(No.2012ZX9301002003)~~

摘　　要：为了考察基因snogE中断对诺加霉素生物合成的影响,比对多种糖基转移酶的氨基酸序列,并克隆与已知蒽环类抗生素糖基转移酶序列同源性最高的snogE编码基因片段,构建基因中断质粒pSXW-2-62。利用接合转移的方法将其转入产诺加霉素的胡桃链霉菌中并发生同源重组,得到重组菌株mSXW-2-71,验证突变株基因型与发酵产物。结果表明,基因中断质粒以正确方式整合入基因组,中断了snogE基因;在突变株发酵产物中无法检测到诺加霉素产生。snogE基因编码的糖基转移酶在诺加霉素的生物合成途径中是必需的。This study investigated the influence of snogE disruption in biosynthesis pathway of nogalamycin. According to the sequence alignment of several glycosytransferases, we cloned the fragment of snogE gene sharing the highest homology with the published anthracycline antibiotics glycosyltransferase sequence. The gene disruption plasmid pSXW-2-62 was constructed and transferred into Streptomyces nogalater ATCC27451 by conjugation. Finally, we got the recombinant strain mSXW-2-71 by homologous recombination. The genotype and fermentation product of mSXW-2-71 were identified. As a result, the gene disruption plasmid was integrated into the genome and interrupted snogE gene successfully. Nogalymycin could not be detected in fermentation broth. The snogE encoding glycosyltransferase is necessary in nogalamycin biosynthesis pathway.

关 键 词：诺加霉素 糖基转移酶 基因中断 同源重组 

分 类 号：R914.5[医药卫生—药物化学]