聚苯乙烯微球吸附脂肪酶催化油酸环氧化的研究  

STUDIES ON EPOXIDATION REACTION OF OLEIC ACID CATALYZED BY LIPASE ADSORBED ON POLYSTYRENE MICROSPHERES

作　　者：樊亚超[1,2] 张松平[1] 马光辉[1] 周炜清[1] 苏志国[1] 张羽飞[1] 

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [2]中国科学院大学,北京100049

出　　处：《离子交换与吸附》2013年第3期200-210,共11页Ion Exchange and Adsorption

基　　金：国家重点基础研究发展规划(973)基金资助项目(No.2009CB724705);国家自然科学基金资助项目(No.20976180;21106164);中科院知识创新项目(No.KSCX-EW-G-3)

摘　　要：南极假丝酵母脂肪酶B(Candida antarctica Lipase B,CALB)在H2O2的存在下可以有效地催化不饱和脂肪酸上双键的环氧化反应,制备出具有广泛用途的环氧植物油和环氧脂肪酸,但反应体系中的H2O2和反应生成的过氧酸对酶有严重的失活作用,需通过固定化提高酶稳定性。本文选用具有不同亲疏水性和孔径的聚苯乙烯微球,通过吸附法固定化CALB,并构建了非水相和油/水两相体系作为CALB催化油酸环氧化反应的介质。结果表明,固定化CALB在非水相体系中具有较高的活力,而在油/水两相体系中重复使用性能较好;CALB在疏水性介孔聚苯乙烯微球PST30上具有最高的酶负载量(100.7mg/g),在疏水性超大孔聚苯乙烯微球PST上表现出最高的催化活力和重复使用稳定性,其在非水相和两相反应体系中的催化活力分别是Novozym 435的1.8倍和7.3倍,重复使用次数与Novozym 435相当,在油/水两相体系中重复使用至第2次还出现独特的活力升高现象。The epoxidation reaction of double bond in unsaturated fatty acid could be effectively catalyzed by Candida Antarctica lipase B （CALB） in the existence of H202, thus producing epoxy vegetable oil and epoxy fatty acids with extensive application prospects, whereas the enzyme CALB was seriously inactivated by the contact with H202 and in situ generated peracid. Therefore, immobilization was necessary to improve enzyme stability. In this study, CALB was adsorbed on polystyrene microspheres with different hydrophobicity and pore sizes, which were then used to catalyze epoxidation reaction of oleic acid in nonaqueous and biphasic system, respectively. The results indicated that immobilized CALB exhibited higher catalysis activity in nonaqueous sysem, but showed better stability in biphasic system. The highest loading amount of 100.7mg/g was obtained when adsorbing CALB on hydrophobic mesoporous PST30 microspheres, while the highest catalysis activity and stability were got by CALB loading on hydrophobic gigaporous PST microspheres. The catalysis activity of CALB-loaded PST microspheres was 1.8 and 7.3 times higher than that of Novozym 435 when catalyzed in nonaqueous and biphasic system separately. In addition, the reusability of CALB-loaded PST microspheres was comparable to that of Novozym 435. It was also worth to be mentioned that the catalysis activity of CALB-loaded PST microspheres tended to be increased during the reuse process in biphasic system.

关 键 词：聚苯乙烯微球 脂肪酶 环氧化 非水相体系 油 水两相体系 

分 类 号：Q814.2[生物学—生物工程]