人参和西洋参特有挥发性成分鉴别  

Analysis of Unique Volatile Components from Ginseng and Quinquefolius Using for Determining

作　　者：佟鹤芳[1] 薛健[1] 童燕玲[1] 刘慧灵[1] 赵保华[1] 

机构地区：[1]北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室,北京100193

出　　处：《中国实验方剂学杂志》2013年第21期120-122,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2009ZX09502-027;2009ZX09308-001-1-5)

摘　　要：目的:对比研究人参和西洋参挥发性成分,探讨用于鉴别的可能性。方法:人参和西洋参分别采用水蒸气蒸馏法、索氏提取法、固相微萃取法和吹扫捕集法得到挥发性成分,运用GC-MS技术分离与检测,所得总离子流图通过NIST谱库检索并结合保留指数(Kovats’RI)对具体化学成分进行分析和鉴定。结果:4种方法提取的人参和西洋参挥发性气味成分种类和含量存在差异,水蒸气蒸馏共鉴定人参样品萜类化合物42个(相对含量69%),西洋参28个(41%);索氏提取共鉴定人参样品萜类化合物26个(50%),西洋参15个(18%);固相微萃取共鉴定人参样品萜类化合物18个(49%),西洋参5个(46%);吹扫捕集共鉴定人参样品萜类化合物2个(4%),西洋参4个(13%)。结论:同一方法条件下人参和西洋参各自具有的特征性成分,可用于辅助区别人参和西洋参。Objective： To identify Ginseng and Quinquefolius by analysis of their volatile components; Method： Ginseng and Quinquefolius volatile components were extracted by Soxhlet, Steam distillation, SPME, Purge and trap extraction, methods, separated and detected by GC -MS, analyzed by searching NIST library and KI value. Result： There are obvious difference in type and content of volatile compounds in Ginseng and Quinquefolius using four different extraction methods. 42 （ relative amount 69% ） and 28 （41%） terpenoid components were identified in Ginseng samples and Quinquefolius samples by steam distillation respectively; 26 （50%） and 15 （ 18% ） terpenoid components were identified in Ginseng samples and Quinquefolius samples by Soxhlet; 18 （49%） and 5 （46%） terpenoid components were identified in Ginseng samples and Quinquefolius samples by SPME respectively; 2 （4%） and 4 （13%） terpenoid components were identified in Ginseng samples, and Quinquefolius samples by purge and trap extraction Conclusion： Characteristic volatile components in Ginseng and Quinquefolius are different under the same extraction conditions. We could use these properties to distinguish between the two herbs.

关 键 词：人参 西洋参 挥发性成分 气相质谱-色谱联用仪 KI 鉴别 

分 类 号：R284.1[医药卫生—中药学]