2个马铃薯杂交组合F_1的SSR鉴定  

Identification of two cross combinations of F_1 Solanum tuberosum by SSR molecular marker

作　　者：张自强[1] 于肖夏[1] 鞠天华 于卓[1] 崔阔澍[1] 王丹[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019 [2]赤峰天泽生物科技有限公司,赤峰025457

出　　处：《中国生态农业学报》2013年第11期1411-1415,共5页Chinese Journal of Eco-Agriculture

基　　金：国家科技支撑计划项目(2012BAD02B05);内蒙古自然科学基金重大项目(2013ZD03);内蒙古马铃薯产业发展重大技术集成创新与示范项目(20131706)资助

摘　　要：杂交育种是马铃薯新品种选育的重要方法,其杂种F1真实性鉴定是获得目标性状单株的关键环节。为选育优质、高产、抗病性及抗旱性强的马铃薯新品种,用马铃薯品种"费乌瑞它"(Favorita)分别与"J07-6"和"陇薯3号"杂交,获得了杂种F1代。本试验利用SSR标记技术对"Favorita"×"J07-6"、"Favorita"×"陇薯3号"2个杂交组合F1共86个单株的真实性进行了鉴定。试验从43对SSR引物中筛选出2对适宜引物STM1049和S7。利用这2对引物进行PCR扩增,将"Favorita"×"J07-6"杂交种F1和"Favorita"×"陇薯3号"杂交种F1的SSR带型划分为双亲互补型、缺失型、父本型和母本型4种类型。依据SSR带型特征,从"Favorita"×"J07-6"和"Favorita"×"陇薯3号"2个杂交组合F1单株中分别鉴定出真杂种34个和27个,SSR分子标记技术用于马铃薯杂交种真实性鉴定是可靠的。该研究结果可为马铃薯杂交种优良株系选育提供依据。Cross breeding has been a major breeding method of potato and F1 hybrid generation authenticity identification critical for objectively obtaining individual plant characteristics. To raise new potato （Solanum tuberosum） varieties with good quality, high yield, strong disease resistance and drought resistance, ＂J07-6＂ and ＂Longshu No.3＂ were respectively cross bred with ＂Favorita＂ and F1 hybrids derived. A total of 86 individual F1 hybrid authenticity of two hybrid combinations of ＂Favorita＂ × ＂J07-6＂ and ＂Favorita＂ × ＂Longshu No.3＂ were identified using the SSR molecular markers. Out of 43 pairs, 2 pairs of SSR primers （STM1049 and S7） were selected and used in marker analysis of the hybrid and parent crops. The banding patterns of ＂Favorita＂ × ＂J07-6＂ and ＂Favorita＂ × ＂Longshu No.3＂ hybrids were divided into four groups - complementary pattern of parents, deletion pattern, male specific pattern and female specific pattern. A total of 34 individual F1 hybrids of ＂Favorita＂ × ＂J07-6＂and 27 individual F1 hybrids of ＂Favorita＂ × ＂Longshu No.3＂ were authentically identified. This showed that SSR molecular marker technology was feasible for potato hybrid authenticity identification. The results of this study laid the needed reference for hybrid breeding of excellent potato strains.

关 键 词：马铃薯 杂种F1 单株 真实性 SSR鉴定 

分 类 号：S158[农业科学—土壤学] S336[农业科学—农业基础科学]