高效液相色谱法测定不同品种苹果芽中根皮苷的含量  

Determination of Phloridzin in Apple Buds from Different Varieties by HPLC-DAD

作　　者：冉军舰[1,2] 樊明涛[1] 赵政阳[3] 梁俊[3] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]河南科技学院食品学院,河南新乡453003 [3]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《食品科学》2013年第20期157-160,共4页Food Science

基　　金：国家现代农业产业技术体系建设专项(nycytx-08-01-03);陕西省科技攻关项目(2010K01-04-1)

摘　　要：建立了检测苹果芽中根皮苷含量的高效液相色谱联合二极管阵列检测器的方法。色谱条件为Diamonsil C18柱(250mm×4.6mm,5μm),柱温30℃,流动相为体积分数1%的乙酸溶液和甲醇,梯度洗脱,检测波长280nm,标准曲线的线性范围6.25～100μg/mL,决定系数0.9999,检测限为0.0313μg/mL,定量限为0.0939μg/mL,回收率范围95.38%～100.12%。检测了富士、国光、乔纳金、粉红女士、秦阳、秦冠、嘎啦、澳洲青苹、红玉、红星10个品种的苹果芽,结果表明:根皮苷含量最高的是红星,含量达到17.284mg/g;秦阳芽中根皮苷的含量最低,为12.569mg/g。A method to detect phloridzin in apple buds was established using high performance liquid chromatography （HPLC） with a diode array detector （DAD）. Chromatographic separation was conducted on a Diamonsil C18 （250 mm × 4.6 mm, particle size 5 μm） with a gradient elution program using a mobile phase made up of 1% acetic acid （V/V） and methanol as at 280 nm. The method had a linear range from 6.25 to 100 μg/mL with correlation coefficient of 0.9999, the limit of detection was 0.0313 μg/mL and the limit of quantification was 0.0939 μg/mL. The recovery ranged from 95.38% to 100.12%. Phloridzin in apple buds from 10 different varieties （Fuji, Ralls, Jonagold, Pink lady, Qinyang, Qinguan, Gala, Granny Smith, Jonathan, Starkrimzon） grown in Baishui county, Shaanxi province were determined by the HPLC method. The results showed that the highest content of phloridzin in Starkrimzon buds was 17.284 mg/g among the varieties studied, and the lowest content in Qinyang was 12.569 mg/g.

关 键 词：苹果芽 根皮苷 高效液相色谱 

分 类 号：S661.1[农业科学—果树学]