ELISA法检测牛奶中头孢氨苄残留  

Development of ELISA Method for Detection of Cepfalexin Residue in Milk

作　　者：卞素敏[1,2] 扈洪波[2] 邢仕歌[1] 张悦[1] 熊齐荣[1] 钟强[1] 金涌[1] 储晓刚[1] 

机构地区：[1]中国检验检疫科学研究院食品安全研究所,北京100123 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2013年第20期186-189,共4页Food Science

基　　金：中国检验检疫科学研究院基本科研业务费专项(2011JK017)

摘　　要：应用头孢氨苄单克隆抗体,建立牛奶中头孢氨苄残留的间接竞争酶联免疫吸附测定(ELISA)检测方法,其牛奶中检测线性范围为1.0～50.0ng/mL,检测限为1.0ng/mL,半数抑制质量浓度为4.1ng/mL,牛奶的加标回收率分别为96.0%、98.2%及93.3%。头孢氨苄抗体与头孢拉定、头孢羟氨苄、头孢克洛及头孢克肟的交叉反应率分别为160.0%、80.0%、28.4%和3.7%。此外,一种简单、有效的牛奶纯化方法得以建立。对比ELISA和超高效液相色谱-串联质谱实验结果,显示两种方法有很好的数据相关性(R2=0.9988)。Based on cafalexin monoclonal antibody, an indirect competitive enzyme-linked immunosorbent assay （ELISA） to determine cefalexin residues in milk was established. The linear detection range of the ELISA method in milk was 1.0 to 50.0 ng/mL, with limit of detection of 1.0 ng/mL, and IC50 value of 4.1 ng/mL. The recovery rates for spiked milk with 50, 100 ng/mL and 200 ng/mL of cafalexin were 96.0%, 98.2% and 93.3%, respectively. Satisfactory cross-reactivity of the antibody with cefradine, cefadroxil, cefaclor and cefixme was also observed （160.0%, 80.0%, 28.4% and 3.7%, respectively）. In addition, a simple and effective sample extraction method was established. The ELISA assay was validated by comparing its results with those obtained by using UPLC-MS-MS, with a good correlation （R2） of 0.9988.

关 键 词：头孢氨苄 单克隆抗体 间接竞争酶联免疫吸附测定 牛奶 残留检测 超高效液相色谱-串联质谱 

分 类 号：R978.11[医药卫生—药品]