应用体外发酵法研究高精料饲粮NSC/NDF与甲烷产量之间的关系  

A Study on Relationship between Dietary NSC/NDF and Methane Production of High Concentrate Diets using in Vitro Incubation Technique

作　　者：郑文思[1] 赵广永[1] 张婷婷[1] 牛文静[1] 董瑞兰[1] 

机构地区：[1]中国农业大学动物科技学院,动物营养学国家重点实验室,北京100193

出　　处：《动物营养学报》2013年第10期2315-2324,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(31072055);国家"十二五"科技支撑计划项目(2011BAD26B03-4)

摘　　要：本试验旨在应用体外发酵法研究高精料饲粮非结构性碳水化合物/中性洗涤纤维(NSC/NDF)与甲烷(CH4)、产量之间的关系。采用常规饲料设计饲粮配方,共设计5个精粗比(60∶40、70∶30、80∶20、90∶10和100∶0),每个精粗比设计10种饲粮,共50种试验饲粮。应用体外产气法对样品进行体外48 h发酵。发酵结束后,收集气体和液体样品,测定气体中CH4和CO2产量及液体中挥发性脂肪酸(VFA)产量。结果表明:饲粮NFC/NDF与体外发酵总产气量、CH4和CO2产量之间均存在极显著的正相关关系(P<0.01),与乙酸/丙酸和CH4/TVFA之间均存在极显著的负相关关系(P<0.01),但是相关系数均偏低。结果提示,高精料饲粮条件下,随着饲粮NSC/NDF的提高,饲粮体外发酵的CH4相对产量下降,但由于相关系数偏低,饲粮NSC/NDF不能作为准确预测饲粮体外发酵CH4产量的指标。This experiment was conducted to study the relationship between dietary non-structural carbohydrates/neutral detergent fiber （NSC/NDF） and methane （CH4）,production of high concentrate diets using in vitro incubation technique.Fifty diets with 5 ratios of concentrate to roughage （60∶40,70∶30,80∶20,90∶10 and 100∶0） and 10 diets for each ratio were formulated.An in vitro gas test was used for 48 h incubation.After that,gas and liquid samples were collected for the determination of CH4 and CO2 production in gas and volatile fatty acids （VFA） production in liquid.The results showed that significantly positive relationships were found between dietary NSC/NDF and CH4,CO2 and total VFA （TVFA） production （P 〈0.01）,and significantly negative relationships were found between dietary NSC/NDF,acetate/propionate and CH4/TVFA （P 〈0.01）,but regression coefficients were all low.It is concluded that in vitro relative CH4 production of high concentrate diet is decreased with the increase of dietary NSC/NDF,but the low regression coefficients indicate that in vitro CH4 production can not be accurately predicted merely based on dietary NSC/NDF.

关 键 词：NFC NDF 体外发酵 甲烷 挥发性脂肪酸 

分 类 号：S816.5[农业科学—饲料科学] S823[农业科学—畜牧学]