葡萄籽提取物原花青素磷脂复合物抗氧化功能的实验研究  

Experimental study on anti-oxidative effect of proanthocyanidins phospholipid complex in grape seed

作　　者：苗智如[1] 袁夏[2] 廖丽娜[2] 侯中海 蒋国军[2] 

机构地区：[1]杭州市萧山区第一人民医院药剂科,浙江杭州311200 [2]浙江萧山医院药剂科,浙江杭州311201

出　　处：《药学实践杂志》2013年第5期362-365,共4页Journal of Pharmaceutical Practice

基　　金：杭州市科学技术局医疗卫生科研项目(20100633B26);杭州市萧山区科学技术局重点科研项目(2010225)

摘　　要：目的探讨葡萄籽提取物原花青素磷脂复合物的抗氧化作用。方法取大鼠80只,随机分为8组:空白对照组、模型组、原花青素组、大豆磷脂组、维生素C组、原花青素磷脂复合物低、中、高剂量组。每天灌胃1次,连续灌胃30 d。然后用50%乙醇给大鼠一次性灌胃,造成乙醇氧化损伤模型。通过测定大鼠体重,血清和肝组织中丙二醛含量、蛋白质羰基含量、超氧化物歧化酶和谷胱甘肽过氧化物酶活性及还原性谷胱甘肽含量的变化,观察葡萄籽提取物原花青素磷脂复合物的抗氧化作用。结果原花青素磷脂复合物各剂量组的大鼠体重与模型组及空白对照组比较,均无统计学差异(P>0.05)。原花青素磷脂复合物低剂量组大鼠血清和肝组织MDA含量明显低于模型组(P<0.05),血清和肝组织GSH-PX活性明显高于模型组(P<0.05)。与模型组比较,原花青素磷脂复合物中剂量组和高剂量组大鼠血清和肝组织中SOD、GSH-PX活性及GSH含量较模型组显著升高(P<0.05,P<0.01),MDA和蛋白质羰基含量明显降低(P<0.05,P<0.01)。结论葡萄籽提取物原花青素磷脂复合物具有显著地抗氧化作用。Objective To study the antioxidant effects of grape seed proanthocyanidins phospholipid complex（GSPPC）. Meth- ods Eighty rats were randomly divided into eight groups： normal control, model control, Proanthocyanidins, Soybean phospholipids, Vi- tamin C and low dose of GSPPC, moderate dose of GSPPC, and high dose of GSPPC. Drugs were given orally to rats once a day for 30 days. And then, 50% ethanol was given to rats once by oral garages to make the ethanol oxidative damage model at the end of experi- ment. The body weight of rats was measured. The contents of MDA, Protein earbonyl （PC） , SOD, GSH and GSH-PX in hepatic tissue and blood serum were detected. Results The results showed there were no significant differences in the body weight among the three GSPPC groups, normal control and model control（P 〉 0.05 ）. Compared with the model control group, the content of MDA in low dosage of the GSPPC group was decreased in blood serum and hepatic tissue（P 〈 0.05 ） , and the content of GSH-PX was increased in blood ser- um and hepatic tissue （P 〈 0.05）. Compared with the model control group, the contents of GSH, SOD and GSH-PX were increased in blood serum and hepatic tissue（P 〈0.05, P 〈0.01 ） , the contents of MDA and PC in middle and high dosage of the GSPPC group were decreased in hepatic tissue and blood serum（P 〈 0.05, P 〈 0.01 ）. Conclusion The GSPPC showed significantly antioxidant effect .

关 键 词：葡萄籽提取物 原花青素 大豆磷脂 抗氧化作用 

分 类 号：R979.5[医药卫生—药品]