携带人血清白蛋白cDNA的逆转录病毒质粒载体的构建  

Construction of a recombination plamid by using new retroviral vector linked cDNA human surem albumin(HSA) gene

作　　者：邱松波[1] 郑新民[1] 曹军平[1] 华文君[1] 乔宪凤[1] 赵浩斌[1] 肖作焕[1] 周荆荣[1] 李莉[1] 魏庆信[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所生物室,武汉430064

出　　处：《湖北农业科学》2000年第5期57-59,共3页Hubei Agricultural Sciences

基　　金：湖北省农业科学院和畜牧兽医研究所自选课题资助

摘　　要：采用粘平端定向基因克隆技术 ,成功地构建了携带HSAcDNA基因片段的重组逆转录病毒质粒载体GINHSAa。经限制性酶切鉴定和菌落原位杂交证明HSAcDNA已克隆到G1Na当中并得到In this article,GINHSAa,a recombination plasmid using new retroviral vector imediated cDNA of HSA was successfully constructed by applying for flush stick and linkage method.It proved by both the restriction endonuclease and bacteria in situ hybridization that cDNA of HSA had already been linked into MCS of the G1Na vector end 9 positive clones were achieved.

关 键 词：生物制品 逆转录病毒 重组人血清白蛋白 载体 

分 类 号：TQ464.7[化学工程—制药化工] Q78[生物学—分子生物学]