HIF-1、MGMT在不同级别胶质瘤中的表达及其与术后化疗疗效的关系  

Study on the relationship between the expression of HIF-1,MGMT and post-operation chemotherapy in various grade glioma

作　　者：邢鹏辉[1] 张学新[1] 李建峰[1] 刘海英[1] 佟静[1] 刘英姿[1] 袁江伟[1] 

机构地区：[1]石家庄河北医科大学第四医院神经外科,050011

出　　处：《疑难病杂志》2013年第11期852-854,F0003,共4页Chinese Journal of Difficult and Complicated Cases

基　　金：河北省医学科学研究重点课题计划项目(No.20120384)

摘　　要：目的探讨缺氧诱导因子(HIF-1)、O^6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)在不同级别胶质瘤的表达及其与化疗效果的关系。方法收集2007年7月一2009年6月手术切除并经病理证实的不同级别胶质瘤患者63例,分为低级别(WHO分级Ⅰ~Ⅱ级)组27例和高级别(WHO分级Ⅲ~Ⅳ)组36例,采用免疫组织化学S-P法检测HIF-1、MGMT蛋白在不同级别胶质瘤中的表达,术后均采取适形放疗,放疗剂量54~60 Gy,根据肿瘤级别及MGMT表达不同给予相同方案化疗,统计患者3年生存率,探求其临床意义。结果 HIF-1、MGMT在低级别胶质瘤中阳性表达分别为13例(48.1%)、11例(40.7%),在高级别胶质瘤中阳性表达分别为29例(80.6%)、27例(75.0%),组间比较差异有统计学意义(P<0.05),同种化疗方案治疗下同级别胶质瘤HIF-1不同表达患者3年生存率差异明显(P<0.05),MGMT不同表达之间因例数较少,未进行统计学分析。结论 HIF-1、MGMT在不同级别胶质瘤的预后评估中具有重要意义,根据HIF-1、MGMT表达不同选择合适方案化疗能明显延长患者生存期。Objective To discuss the relationship between the expression levels of HIF-1,MGMT and post-operation chemotherapy in various grade glioma.Methods Immunohistochemistry S-P methods were undertaken to test HIF-1 and MGMT proteins expression in samples of 63 various grades glioma patients which were proved by histology from July 2007 to June2009.63 patients were divided into two groups which were low grade and high grade groups.Conformal radiotherapy with 54-60 Gy radiation dose and different chemotherapy methods were employed according to the different tumor grade and MGMT expressions.Three year survival rates were observed to analyze the clinical significance.Results HIF-1 positive expressions were observed in 13 of 27 in low grade gliomas（WHO grade Ⅰ-Ⅱ,48.1%） and 29 in 36 of high grade gliomas（WHO gradeⅢ-Ⅳ,80.6%）.While,MGMT positive expressions were observed in 11 of27（40.7%） in low grade giomas and 27 in 36（75.0%） in high grade gliomas.HIF-1 and MGMT co-positive expressions were found in 7（25.9%） in low grade and 23（63.9%） in high grade glioma patients.Three year survival rates had significant difference in same grade glioma patients by the same treatment（P 0.05）.Conclusion HEF-l and MGMT play important role in the prognosis evaluation in various grade gliomas.Choosing optimal chemotherapy regimen for glioma patients according to HIF-1 and MGMT expression can benefit for prolonging survival.

关 键 词：胶质瘤 HIF-1 MGMT 免疫组化 预后 

分 类 号：R739.4[医药卫生—肿瘤]