EB病毒Rta-IgG定量检测试剂盒分析性能评估及其在鼻咽癌血清诊断学中的应用  

Performance evaluation of EB virus Rta-IgG quantitative detection kit and its application in diagnosing nasopharyngeal carcinomathe

作　　者：覃桂芳[1] 李友琼[1] 卢秋维[1] 罗焰芳[1] 夏莉莉[1] 

机构地区：[1]广西壮族自治区人民医院检验科,南宁530021

出　　处：《中国临床新医学》2013年第10期942-944,共3页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：广西卫生厅科研课题(编号:桂卫Z2010248)

摘　　要：目的探讨EB病毒Rta蛋白IgG抗体在鼻咽癌诊断中的临床价值。方法采用间接ELISA法检测612例临床样本中EB病毒Rta-IgG含量,同时对试剂盒的分析性能进行评估。结果 267例鼻咽癌样本的EB病毒Rta-IgG抗体浓度为(77.64±3.205)U/ml,检测灵敏度为94.7%(214/226),特异度为86.3%(333/386),正确率为89.4%(547/612);255例健康体检血样抗体浓度为(8.31±0.745)U/ml;20例肝癌样本抗体浓度为(7.14±1.111)U/ml;30例肝炎样本抗体浓度为(7.40±0.581)U/ml;20例自身免疫病样本抗体浓度为(13.97±6.943)U/ml;20例鼻咽息肉样本抗体浓度为(12.58±6.197)U/ml。各组EB病毒Rta-IgG含量比较差异有统计学意义(P<0.05)。结论 EB病毒Rta-IgG定量检测试剂盒用于鼻咽癌诊断具有较高的临床价值,为进一步研究鼻咽癌患者治疗效果与患者血清中EB病毒Rta-IgG的含量的关系奠定了基础。Objective To investigate the clinical value of Epstein-Barr virus （EBV） Rta/IgG in the diagno- sis of nasopharyngeal carcinoma（NPC）. Methods Sera from 612 clinical samples were measured for the Rta-IgG by an indirect enzyme-linked immunosorbent assay. In the present study, we also evaluated the performance of the Rta- IgG ELISA kits. Results The mean value of Rta-IgG concentration in 267 NPC patients was （77.64 ±3. 205） U/ml with a sensitivity of 94. 7% （214/226） and specificity of 86.3% （333/386） and accuracy of 89. 4% （547/612）. The mean value of Rta-IgG concentration in 255 healthy volunteers ,20 hepatoeellular carcinoma patients ,30 hepatitis patients ,20 patients with autoimmune disease and 20 patients with nasopharyngeal polyp were （8. 31± 0. 745 ） U/ml, （7.14 ±1. 111）U/ml, （7.40±0.581）U/ml, （13.97 ±6.943）U/m1 and （12.58 ±6. 197）U/m1, respectively. There were significant difference between NPC group and other groups. Conclusion Our results Suggest that the EBV Rta/IgG ELISA kit is suitable for the clinical diagnosis of NPC. It also provides a basis for further study on the rela- tionship between the therapeutic effect and serum EBV Rta-lgG content in NPC patients.

关 键 词：EB病毒Rta—IgG 定量检测 鼻咽癌 诊断 

分 类 号：R730.4[医药卫生—肿瘤]