尿清蛋白质控品的研制及室间质评的调查  

Development of urinary albumin quality control materials and investigation of external quality assessment

作　　者：崔小璠[1] 刘蕊[1] 杨彬 

机构地区：[1]天津市人民医院,300121 [2]天津市临检中心,300192

出　　处：《检验医学与临床》2013年第20期2671-2672,共2页Laboratory Medicine and Clinic

摘　　要：目的制备尿清蛋白室内质控品,并进行室间质评调查。方法选取患者尿液样本,离心取上清液,加入乙二醇作为稳定剂,叠氮钠作为防腐剂,分装保存,观察稳定性和瓶间差,并进行室间质评调查。结果自制的尿清蛋白质控品除4℃保存的低值质控品外,CV均小于10%,有较好的稳定性,尤其在前3个月;此外,自制质控品的瓶间差较小,高值质控品瓶间差仅0.67%和0.81%,同时质控品的保存温度对瓶间差的影响很小。在全市的室间质评调查中低值质控品CV为63.3%(29.1%～70.3%);高值质控品CV为20.5%(15.9%～22.3%)。结论研制的尿清蛋白质控品的稳定性较好,易于制备,可应用于临床实验室的室内质控和室间质评。Objective To prepare internal quality control（QC） materials of urinary albumin and to investigate the external quality assessment .Methods Urine samples from patients were centrifuged to obtain supernatant ,and QC materials were prepared by adding ethylene glycol as stabilizer and sodium azide as preservative .QC materials were packaged and preserved ,and stability and between-bottles difference were analyzed .Then external quality as-sessment was performed among clinical laboratories .Results All QC materials ,except low-value QC materials pre-served at 4 ℃ ,were with low CV value（less than 10% ） and with fine stability .The between-bottles difference was small and not obviously influenced by preservation temperature ,and the differences between high-value QC materials were 0 .67% and 0 .81% .External quality assessment indicated that the CV values of low-and high-value QC materi-als were 63 .3% （29 .1% -70 .3% ） and 20 .5% （15 .9% -22 .3% ） .Conclusion The stability of self-prepared QC ma-terials of urinary albumin could be fine ,which could be utilized for internal QC and external quality assessment .

关 键 词：尿清蛋白 质控品 稳定性 瓶间差 室间质评 

分 类 号：R446[医药卫生—诊断学]