分光光度法测定畜禽骨素中胶原蛋白含量  

Determination of Collagen Content in Livestock and Poultry Ossein by Spectrophotometry

作　　者：董宪兵[1] 李侠[1] 张春晖[1] 孙红梅[1] 李银[1] 

机构地区：[1]中国农业科学院农产品加工研究所/农业部农产品加工重点实验室,北京100193

出　　处：《现代食品科技》2013年第10期2538-2541,2419,共5页Modern Food Science and Technology

基　　金：农业部948项目(2011G8);国家科技合作与交流专项(010S2012ZR0302);中央级院所创新项目(2012EG134238);中央级公益性科研院所基本科研业务费专项(2012ZL034)

摘　　要：本文提供了一种快速、简单、准确、成本低的畜禽骨素中胶原蛋白含量的测定方法。通过热法和酸法提取畜禽骨中胶原蛋白，计算其中胶原蛋白与羟脯氨酸含量的比值，得到不同原料骨的胶原蛋白换算系数：猪骨为6.12-6.17，牛骨为6.77，鸡骨为6.27-6.30，羊骨为6.34-6.35，结果表明水溶性和酸溶性的胶原蛋白换算系数差异不显著（P〉0.05）。根据骨素生产实际情况,选用水溶性胶原蛋白换算系数作为骨素中胶原蛋白测定的换算系数，用分光光度法测定热提骨素中羟脯氨酸含量，乘以对应的胶原蛋白换算系数，得到猪骨素、牛骨素和鸡骨素中胶原蛋白含量分别为9.19%、7.92%、9.78%。该方法测定骨素中胶原蛋白含量的相对标准偏差在0.01-0.03%之间，变异系数在0.44-2.26%之间，平均回收率在99.36-100.44%之间，说明分光光度法适用于骨素中胶原蛋白含量的测定。The paper provided a rapid, simple, accurate and low cost method to determine collagen content in ossein. Collagens from livestock and poultry bone were extracted by acid and thermal processes, and then calculated the ratio of collagen protein and hydroxyproline content in order to obtain collagen conversion factors from different bones. However the results indicated that fewer differences of collagen conversion factors between water-soluble and acid soluble collagen could be seen. According to the actual production, water-soluble collagen was taken as the conversion factor in ossein. The hydroxyproline in ossein was measured by spectrophotometry and then the collagen contents of pig, bovine and chicken ossein were calculated as 9.19%, 7.92% and 9.78%, respectively. The relative standard deviation （RSD） was between 0.01% and 0.03% and coefficient variation （CV） changed from 0.44% to 2.26%. The average recovery rate ranged from 99.36% to 100.44%. In conclusion, the method was appropriate to detect of collagen content in livestock and poultry ossein.

关 键 词：分光光度法 畜禽骨素 胶原蛋白换算系数 羟脯氨酸 胶原蛋白 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程] O657.3[轻工技术与工程—食品科学与工程]