马传染性贫血病毒驴白细胞弱毒疫苗gag基因的序列测定及分析  

Sequencing and Analysis of gag Gene of Donkey Leukocyte Attenuated Equine Infectious Anemia Virus

作　　者：杨威[1] 张宝山[2] 刘永刚[2] 刘胜旺[2] 孔宪刚[2] 曹殿军[2] 刘相冬[3] 卢景良[2] 刘宝全[1] 刘建华[2] 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001 [3]哈尔滨医科大学公共卫生学院,黑龙江哈尔滨150001

出　　处：《病毒学报》2000年第4期361-364,共4页Chinese Journal of Virology

基　　金：国家自然科学资助!项目 (39770 5 6 1)

摘　　要：以马传染性贫血病毒 (EIAV)驴白细胞弱毒疫苗株 (DLA)病毒基因组RNA为材料 ,用RT PCR方法扩增出EIAVgag基因 ,经平端连接将其克隆到质粒载体 pUC19中。由于疫苗不是克隆株 ,因此通过 5次单独克隆与测序 ,推导出EIAV DLA gag基因的优势序列。gag基因全长 145 8个碱基 ,编码一 486个氨基酸残基的前体蛋白。与美国EIAVWyoming 136 9株比较 ,核苷酸同源性为 80 % ,氨基酸同源性为 84 47%。其中 ,衣壳蛋白P2 6的主要同源区 (MHR)、核衣壳蛋白P11的两个CCHC型锌指结构及酸性蛋白P9的YXXL基元为高度保守的结构。为进一步研究马传染性贫血病毒弱毒疫苗的致弱机理提供了理论依据。In order to study the attenuated mechanism of donkey leukocyte attenuated (DLA) equine infectious anemia virus (EIAV), the genome RNA of DLA EIAV was used to amplify the gag gene by RT PCR, and the PCR product was cloned to the plasmid pUC19. The cloned gag gene originated from DLA EIAV RNA was further cloned and sequenced for another five times. The resultant DLA EIAV gag gene includes 1,458 bp, which encodes a 486 amino acid precursor protein. The DLA EIAV gag shared the homology of 80% in nucleotide and 84.47% in amino acid with the EIAV Wyoming 1369 strain. Our results demonstrated that there are highly conserved structures in the major homology region (MHR) of capsid protein P26, two CCHC type zinc fingers of nucleocapsid P11 and the YXXL motif in the P9 between DLA EIAV and Wyoming 1369.

关 键 词：马传染性贫血病毒 驴白细胞弱毒 核苷酸序列 

分 类 号：S852.659.3[农业科学—基础兽医学] S858.222.6[农业科学—兽医学]