2011~2012年辽宁地区猪繁殖与呼吸综合征病毒NSP2基因遗传演化分析  

NSP2 genetic evolution analysis of porcine reproductive and respiratory syndrome virus in swine herds in Liaoning Province from 2011 to 2012 years

作　　者：于学武[1,2] 顾贵波[1,2] 陈瑶[1,2] 申贯男[1,2] 吴运谱[1,2] 关淼[1,2] 闫海滨[1,2] 王宏燕[1,2] 兰德松[1,2] 李蓉[1,2] 

机构地区：[1]辽宁省动物疫病预防控制中心 [2]辽宁省动物医学研究院,辽宁沈阳110164

出　　处：《现代畜牧兽医》2013年第10期29-33,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：辽宁省科技厅农业攻关计划项目<辽宁省重大动物疫病病原变异与综合防控技术研究>;项目编号:2011214001

摘　　要：本研究应用分子生物学技术,扩增并测定了2011~2012年辽宁地区6株PRRSV NSP2基因全序列。结果发现,6株PRRSV的NSP2基因全长均为2 850 bp,均于1 143~1 441 bp和1 593~1689 bp发生连续的碱基缺失,且均为变异毒株,具有典型HP-PRRSV的分子特征;同源性与进化分析表明,HP-PRRS在辽宁地区有不同的毒株同时流行,辽宁地区HP-PRRSV可能起源于同一祖先。In this study, molecular biology techniques were used to amplify and se-quencing six strains PRRSV NSP2 genome sequences in swine herds in Liaoning Prov-ince from 2011 to 2012 years. It was found that full-length of six strains PRRSV NSP2 genome sequences were all 2 850 bp. continuous base deletion occurred at 1 441bp^1 143bp and 1 593bp^1 689bp of the six strains PRRSV NSP2 genome;and they were all variant strains. All of he six strains PRRSV NSP2 genome had typical HP-PRRSV molecular characteristics.The analysis of homology and phylogenetic showed that different strains of HP-PRRSV were prevalent in in swine herds in Liaon-ing Province at the same time. The strains of HP-PRRSV may originate from the same ancestor PRRSV strain in swine herds in Liaoning Province.

关 键 词：辽宁 猪繁殖与呼吸综合征 NSP2 遗传演化 

分 类 号：S858[农业科学—临床兽医学]