神经节苷脂对脑缺血/再灌注损伤的保护作用  

Protective Effect of Ganglioside on Cerebral Ischemia/Reperfusion

作　　者：刘松岩[1] 于挺敏[1] 赵力[1] 刘淑玲[2] 李秀芝[2] 迟春萍[2] 王妍[2] 

机构地区：[1]白求恩医科大学第三临床学院,长春130031 [2]长春生物制品研究所,长春130062

出　　处：《中国生物制品学杂志》2000年第1期40-41,共2页Chinese Journal of Biologicals

摘　　要：目的探讨神经节苷脂对脑缺血再灌注损伤的保护作用。方法采用Ｐｕｌｓｉｎｅｌｌｉ的四血管闭塞法 制作大鼠急性脑缺血／再灌注模型，利用高压液相色谱法观察脑缺血／再灌注期间谷氨酸和γ－氨基丁酸含量的变化， 通过图象分析仪测定神经元的面数密度及神经节苷脂对以上指标的影响。结果缺血后谷氨酸和γ－氨基丁酸含 量明显升高，再灌注后降低，神经节苷脂可降低谷氨酸含量，与盐水对照组相比差异显著（Ｐ＜０．０１），同时增加神经 元面数密度。结论神经节苷脂通过抑制谷氨酸释放，可减轻脑缺血再灌注期间脑组织损伤。Objective To explore the protective effect of ganglioside on cerebral ischemia/reperfusion. Methods The rat model of cerebral ischemia/reperfusion was established by four-vessel obstruct method. The changes of glutamic acid and γ-aminobutyric acid contents during cerebral ischemia/reperfusion were observed by HPLC,and the denisity of neuron and the influence of ganglioside on the above-mentioned indexes were analyzed by image pattern analyzer.Results The contents of glutamicc acid and aminobutyric acid were significantly increased after cerebral ischemia, and decreased after reperfusionn. Compared with normal saline, ganglioside can significantly decrease glutamic acid content(P <0.01) and increase neuron denisity.Conclusion Ganglioside can ease the cerebral tissue lesion during cerebral ischemia/reperfusion by inhibiting the release of glutamic acid.

关 键 词：脑缺血/再灌注 谷氨酸 神经节苷脂 保护作用 

分 类 号：R743.3[医药卫生—神经病学与精神病学]