香蕉MADS-Box基因家族的生物信息学分析  

Bioinformatics Analysis of the MADS-Box Gene Family in Banana

作　　者：张远森[1] 秦晓萌[1] 严金平[1] 张昆林[1] 杨恩[1] 

机构地区：[1]昆明理工大学生命科学与技术学院,昆明650500

出　　处：《植物生理学报》2013年第10期1030-1040,共11页Plant Physiology Journal

基　　金：云南省昆明理工大学人才培养项目(14118480)

摘　　要：MADS-box基因家族是具有多种生物功能的转录因子,广泛参与植物的生长发育过程以及逆境应答。为了探索MADS-box基因家族在香蕉果实发育到后熟衰老过程中的功能,本文对71个香蕉MADS-box基因家族基因结构、蛋白的基本理化性质、二级结构预测、亚细胞定位、保守基序等方面进行初步生物信息学分析,结果显示:13个香蕉MADS-box基因没有内含子,5个有较长的上游序列;71个香蕉MADS-box蛋白中有52个偏碱性或者强碱性,8个显中性,11个偏酸性;除5个以无规则卷曲为主要构成元件外,香蕉MADS-box蛋白主要以α-螺旋为主要构成元件;大多数MADS-box蛋白定位于细胞核和线粒体基质,少许分布在其他细胞器;基序1(MADS domain)是该家族的保守基序;此外,进化分析发现,香蕉MADS-box基因家族基因主要是II型,I型仅有6个。另外,现有香蕉MADS-box基因家族中,只有37个可查EST序列。The MADS-box gene family is a mulit-functional transcriptional factor. It is widely involved in plant growth and developmental processes and stress responses. In older to study the function of MADS-box gene in banana fruit from growth to senescence, the gene structure, EST, physicochemical property, secondary structure, subcellular location, conserved motif and other basic properties of banana MADS-box gene family were analyzed by the bioinformatics methods. The results showed that 13 members of MADS family genes had no intron and 5 members just had a upstream. There were 52 alkalinity or alkali proteins, 8 neuter proteins and 1 l acidic proteins in the MADS family. And most of MADS-box proteins located in nucleus and mitochondrial matrix. The secondary structure of most MADS-box proteins was Alpha helix; only 5 of them was random coil. The analysis of motifs predicted that motif 1 （MADS domain） is the conserved motif of banana MADS-box gene family. Furthermore, from the phylogenetic analysis found that only 6 genes belongs to type I, other genes belongs to type II. Moreover, only 37 members ofMADS gene family can inauirv the EST sequences.

关 键 词：香蕉 MADS—box基因家族 生物信息学 

分 类 号：S668.1[农业科学—果树学]