适当浓度的过氧化氢诱导人支气管上皮细胞发生钙振荡(英文)  

Moderate Concentration of H_2O_2 Induced [Ca^(2+)]_i Oscillation in Human Bronchial Epithelial Cells

作　　者：杨卫兵[1] 王永全[1] 叶青[2] 蔡鹏程[3] 朱莉萍[4] 叶红[4] 马万里[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院呼吸内科,武汉430022 [2]怀化学院计算机工程系,怀化418000 [3]华中科技大学同济医学院附属协和医院检验科,武汉430022 [4]华中科技大学同济医学院病理生理系,武汉430030

出　　处：《中国细胞生物学学报》2013年第10期1485-1491,共7页Chinese Journal of Cell Biology

基　　金：supported by the National Natural Science Foundation of China(Grant No.81200020,30770648)~~

摘　　要：包括过氧化氢(H2O2)在内的活性氧通过引起细胞内钙的变化而造成细胞损伤。然而,不同浓度的H2O2可以导致细胞内不同的钙变化,并激活不同的信号通路。细胞内钙振荡是其中的一种钙信号变化形式,钙振荡可以调控转录因子NF-κB的活性。该研究探讨可以诱导支气管上皮细胞内钙振荡发生的H2O2浓度。体外培养人支气管上皮细胞,采取钙离子荧光探针Fura-2标记细胞,并使用离子成像系统,观测不同浓度的H2O2(0～1 000μmol/L)作用下细胞内钙浓度的变化。结果发现,低于50μmol/L的H2O2仅仅引起"钙火花";50～500μmol/L的H2O2导致细胞内钙振荡的发生;而1 000μmol/L的H2O2引起细胞内持续的高钙;同时也证实150μmol/L的H2O2诱发明显的钙振荡,而钙振荡随后引起了NF-κB活性的升高。该研究提示,适当浓度的H2O2可以诱发支气管上皮细胞内钙振荡的发生,推测可能是活性氧导致慢性气道炎症损伤的一个机制。Reactive oxygen species （ROS） including hydrogen peroxide （H202） can damage cells by inducing changes of cytosolic Ca2＋ （[Ca2＋]0. However, different concentration of H202 induced different changes in [Ca2＋]i which activated different signaling pathways. One type of [Ca2＋]i changing is [Ca2＋]i oscillation which can regulate the activity of NF-nB. The aim of this study is to get the concentrations of H202 which can induce [Ca2＋]i oscillation in the bronchial epithelial cells. Human bronchial epithelial cells were cultured, and treated without or with different concentration of H202 （0-1 000 μmoFL）. Cytosolic Ca〉 concentration was detected by fluorescent Ca〉 indicator Fura-2. The results revealed that H202 concentrations 〈50 μmol/Ljust induced [Ca2＋]i spark, 50-500 ~tmol/L induced [Ca2＋]i oscil- lation, and 1 000 μmol/L induced constantly high level of [Ca2＋]i. Moreover, the findings confirmed that 150 μmol/L H202 induced [Ca2＋]i oscillation, and the [Ca2＋]i oscillation induced increases of NF-ld3 activity. In conclusion, these data suggested that moderate concentration （50-500 μmol/L） of H202 can induce [Ca2＋]i oscillation and NF-kB acti- vation in bronchial epithelial cells, which should be involved in ROS induced damage in chronic airway inflamma- tion.

关 键 词：活性氧 过氧化氢 钙振荡 支气管上皮细胞 

分 类 号：R363[医药卫生—病理学]