0.1 Gy X射线照射人淋巴母细胞基因表达转录谱的研究  

Effect of 0.1 Gy X-ray irradiation on gene expression profiles in normal human lymphoblastoid cells

作　　者：李洁清[1] 侯殿俊[1] 马娅[1] 封丽[1] 李卫国[1] 刘伟[1] 乔建维[1] 贾喜明[1] 李坤[1] 

机构地区：[1]山东省医学科学院放射医学研究所,济南250062

出　　处：《中华劳动卫生职业病杂志》2013年第10期749-752,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：山东省自然科学基金（ZR2010CM045）;山东省医药卫生发展计划（2009HZ078）

摘　　要：目的 应用基因芯片分析0.1 GyX射线照射对正常人淋巴细胞基因表达的影响,探讨低剂量辐射生物效应的作用机制.方法 采用含有45033个基因的NimbleGen 12×135K芯片分析0.1 GyX射线照射AHH-1细胞后培养6h和20 h基因表达谱的改变,照射组与对照组细胞的基因表达量差异比值大于2或小于0.5的基因确定为有效差异表达基因.用RT-PCR和real-time PCR方法验证部分差异表达基因.结果 0.1 Gy照射后6h,760个基因表达上调,1222个基因表达下调；照射后20 h,上调和下调基因分别是463和753个,共同差异表达的基因有92个.RT-PCR和real-time PCR方法验证了GADD45A、CDKN2A和Cx43的表达与基因芯片结果一致.结论 低剂量辐射对多种功能基因的表达产生了影响,可以为辐射损伤机制的研究提供依据.Objective To investigate the effect of 0.1 Gy X-ray irradiation on the gene expression profiles in normal human lymphoblastoid cells using gene microarray and to explore the possible mechanism of the biological effect of low-dose irradiation.Methods The NimbleGen 12 ×135 K microarray corresponding to 45033 genes was used to analyze the gene expression profiles in AHH-1 cells cultured for 6 h and 20 h after 0.1 Gy X-ray irradiation.A gene was identified as the differentially expressed gene if the ratio between its expression levels in irradiation group and control group was higher than 2 or lower than 0.5.RT-PCR and real-time PCR were used to confirm some differentially expressed genes.Results There were 760 up-regulated genes and 1222 down-regulated genes in the cells at 6 h after 0.1 Gy X-ray irradiation,while there were 463 up-regulated genes and 753 down-regulated genes at 20 h after 0.1 Gy X-ray irradiation; there were 92 differentially expressed genes in common.The expression of GADD45A,CDKN2A,and Cx43 measured using gene microarray was confirmed by RT-PCR and real-time PCR.Conclusion Low-dose irradiation can affect the expression of many functional genes,which provides a basis for the research on the mechanism of radiation damage.

关 键 词：电离辐射 基因芯片 基因表达谱 差异表达基因 

分 类 号：R363[医药卫生—病理学]