快速周期伏安法在定量研究脑内核团多巴胺释放中的应用  

MONITORING DOPAMINE RELEASE BY FAST CYCLIC VOLTAMMETRY:AN IN VIVO STUDY

作　　者：谢俊霞[1] 周宇[2] 姜宏[1] 

机构地区：[1]青岛大学医学院生理教研室,青岛266021 [2]上海中科院上海生理研究所,上海200031

出　　处：《中国应用生理学杂志》2000年第4期370-372,共3页Chinese Journal of Applied Physiology

基　　金：国家"九五"科技攻关计划!(969060 5 0 8);卫生部优秀青年科技人才专项科研基金资助课题!(96Q 0 85 )

摘　　要：目的和方法 :采用快速周期伏安法 (FCV)在体研究电刺激内侧前脑束 (MFB)或腹侧背盖区 (VTA)诱发的纹状体 (CPu)、伏核 (Acb)或中央杏仁核 (CAN)多巴胺 (DA)释放的特点 ,探索电刺激诱发不同核团DA释放的适宜刺激参数。结果 :CPu、Acb与CAN的DA释放量及释放动力学特征均有不同。结论 :在应用FCV技术研究脑内不同部位DA释放时 ,应重视适宜刺激参数的选择及运用 。Aim and Methods: Using fast cyclic voltammetry (FCV) and carbon fibre microelectrode (CFMe), we monitored dopamine(DA) release from caudate putamen(CPu) ,nucleus accumbens(Acb) and central amygdaloid nucleus(CAN) of normal rats in vivo , in order to find out the suitable parameters to evoke DA release of certain area. DA release was evoked by electrical stimulation of medial forebrain bundle(MFB) or ventral tegmental area(VTA). Results: DA release from CPu, Acb or CAN were different in contents and the characteristics of dynamics. Conclusion: Different parameters should be used according to the different sites of stimulation in order to get the maximal DA release during the experiment.

关 键 词：快速周期伏安法 电刺激 多巴胺 定量研究 释放 

分 类 号：R741.044[医药卫生—神经病学与精神病学] R338.8[医药卫生—临床医学]