ZnSO_4对变异链球菌生长及生物膜形成影响的研究  

Effect of ZnSO_4 on Streptococci mutans proliferation and biofilm formation

作　　者：杨旸[1,2] 江雪 彭文涛 邹岩[1,2] 朱彩莲[4] 何智妍[4] 叶冬霞[4] 唐子圣[1,2] 

机构地区：[1]上海交通大学医学院附属第九人民医院.牙体牙髓科 [2]上海市口腔医学重点实验室,上海200011 [3]联合利华中国投资有限公司,上海200335 [4]上海交通大学医学院附属第九人民医院.口腔医学研究所,上海200011

出　　处：《牙体牙髓牙周病学杂志》2013年第10期613-617,632,共6页Chinese Journal of Conservative Dentistry

基　　金：国家重点基础研究发展计划资助项目(2007CB936004);上海交通大学"医工(理)交叉研究"基金资助项目(YG2010MS61)

摘　　要：目的：观察不同浓度ZnSO4对浮游状态变异链球菌生长、产酸及其生物膜形成的影响.方法：将变异链球菌UA159分别接种于含ZnSO40、1、2、5、10 mmol/L的BHI培养液中,37℃、厌氧培养.分别于培养4、8、12、18、24h检测各组培养液的OD值、pH值,并进行CFU（Colony-Forming Units）计数.同时,将变异链球菌分别于上述各浓度ZnSO4共同培养24 h,使之在盖玻片上形成生物膜；采用CUF计数法观察各组生物膜中细菌数,并运用激光共聚焦扫描显微镜（CLSM）观察各组生物膜中活菌数及其厚度.实验数据用t检验法进行统计分析.结果：1～10 mmoL/L不同浓度ZnSO4均能抑制浮游态变异链球菌的生长、产酸；并使其生物膜变薄,活菌数减少,且其作用随ZnSO4浓度的增加而增加,各组间差异均有统计学意义（P＜0.05）.但是,虽然ZnSO4的浓度为10 mmol/L时能使变异链球菌处于生长停滞状态,却仍有一定的产酸能力,且生物膜内的细菌也未被全部杀死.结论：ZnSO4仅能抑制变异链球菌的生长,无法将其彻底杀灭并阻断其产酸.AIM：To investigate the effect of ZnSO4 on the proliferation,acid production and biofilm formation of streptococci mutans （S.mutans）.METHODS：S.mutans were anaerobicly cultured in BHI containing ZnSO4 at 0,1,2,5 and 10mmoL/L,for 4,8,12,18 and 24 h,respectively.The pH of the culture suspensions was measured and colony-forming unit （CFU） of S.mutans in the suspensions was examined by UV spectrophotometer at OD600.S.mutans biofilms were formed by culture on glass cover slips and CFU in the biofilms was examined.The biofilms were observed by confocal laser scanning microscope （CLSM）.The percentage of vital bacteria was calculated.Data were assessed by SAS8.02 using Student＇s test （t test）.RESULTS：ZnSO4 at higher concentration showed stronger antibacterial effects on S.mutans in both suspension and biofilms （P ＜ 0.05）.10 mmoL/L ZnSO4 nearly completely stopped the proliferation of S.mutans in suspension,but the inhibition of acid production was not complete.The mean thickness of biofilm was reduced significantly,but 10 ～ 20％ bacteria were still alive in the biofilm.CONCLUSION：ZnSO4 to S.mutans is bacteriostasis instead of bactericidal.ZnSO4 should be mantained at high concentration to exert effective antibacterial effects.

关 键 词：变异链球菌 ZnSO4 生物膜 激光共聚焦扫描显微镜 

分 类 号：R780.2[医药卫生—口腔医学]