跨种属肝癌组织细胞周期蛋白B1表达意义研究  

Expression and significance of Cyclin B1 in hepatocellular carcinoma by cross-species research

作　　者：卢晓旭[1] 曹骥[1] 朱伶群[1] 杨春[1] 李瑗[1] 

机构地区：[1]广西壮族自治区肿瘤防治研究所实验研究部,广西南宁530021

出　　处：《中华肿瘤防治杂志》2013年第20期1549-1552,1560,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30960428)

摘　　要：目的:研究细胞周期通路中的细胞周期蛋白B1(Cyclin B1)在人、大鼠、树鼩不同种属的肝癌、癌旁及正常肝组织中的表达和意义。方法:采用实时荧光定量-PCR(Q-PCR)和蛋白质印迹法检测人(38例肝癌及对应癌旁组织、10例正常肝组织)、大鼠(15例肝癌及其相应癌旁组织、14例正常肝组织)和树鼩(10例肝癌及其相应癌旁组织、10例正常肝组织)中Cyclin B1的mRNA和蛋白表达水平。结果:人肝癌组织Cyclin B1mRNA的表达量(0.011 4±0.009 6)高于正常肝组织(0.004 8±0.002 9),差异有统计学意义,P=0.033;高于癌旁组织Cyclin B1mRNA的表达量(0.003 7±0.002 7),差异有统计学意义,P=0.002;癌旁组织与正常组差异无统计学意义,P=0.609。大鼠肝癌组织Cyclin B1mRNA的表达量(0.002 8±0.001 9)高于正常肝组织(0.000 6±0.000 3),差异有统计学意义,P=0.003;其对应的癌旁组织Cyclin B1mRNA的表达量(0.002 9±0.002 5)高于正常肝组织,差异有统计学意义,P=0.001;肝癌组织与其对应的癌旁组织差异无统计学意义,P=0.809。树鼩肝癌组织Cyclin B1mRNA的表达量(0.014 8±0.010 9)高于正常肝组织(0.001 3±0.000 9),差异有统计学意义,P=0.007;对应癌旁组织Cyclin B1mRNA的表达量(0.008 3±0.006 4)高于正常肝组织,P=0.060;肝癌组织与对应的癌旁组织比较,差异无统计学意义,P=0.352。Cyclin B1mRNA在人肝癌组织中的表达与临床分期、门脉癌栓、术后复发、肿瘤直径及肿瘤分化明显有关,而与肝外转移、肿瘤个数及血清AFP水平无明显关系。Cyclin B1蛋白在人的肝癌组织中的平均表达量(0.570 7±0.221 9)明显高于其对应癌旁组织(0.418 5±0.254 3),差异有统计学意义,P=0.005;高于正常肝组织(0.375 4±0.127 0),差异有统计学意义,P=0.019。Cyclin B1在大鼠的肝癌组织中的平均表达量(0.661 9±0.398 1)明显高于正常肝组织(0.372 6±0.104 8),差异有统计学意义,P=0.039。Cyclin B1在树鼩的肝癌组织中的平均表达量(0.656 OBJECTIVE：To study the expression of Cyclin Bl（Ccnbl） in hepatocellular carcinoma （HCC） of differ ent species including human, rat and tree shrew （tupaia）, and to verify the feasibility of cross-species oncogenomics ap-proach. METHODS： Real-time fluorescent quantitative polymerase chain reaction （Q-PCR） and Western Blot were applied to detect the expression levels of Cyelin B1 mRNA and protein levels in human（38 human HCC and Para-HCC tissues and 10 normal liver tissues）,rat（15 HCC and Para-HCC tissues and 14 normal liver tissues）,tree shrew（10 human HCC and para-HCC tissues and normal liver tissues）,respectively. RESULTS： The expression of Cyelin B1 mRNA in HCC tissues of human（0. 011 4±0. 009 6） was significantly higher than that in normal liver tissues（0. 004 8＋0. 002 9,P=0. 033） ; while in HCC adjacent tissues of human（0. 003 7± 0. 002 7） was significantly lower than that in HCC tissues （P = 0. 002）. There was no significant difference between HCC-adjacent tissues and normal liver tissues （P=0. 609）. The ex- pression of Cyclin B1 mRNA in HCC tissues of rat（0. 002 8±0. 001 9） was significantly higher than that in normal liver tissues（0. 000 6 ± 0. 000 3, P= 0. 003） ; while in HCC-adjaeent tissues of human（0. 002 9 ± 0. 002 5） was significantly higher than that in normal liver tissues （P= 0. 001）. There was no significant difference between HCC-adjacent tissues and HCC tissues （P=0. 809）. The expression of Cyclin B1 mRNA in HCC tissues of tree shew（0. 014 8±0. 010 9） wassignificantly higher than that in normal liver tissues（0. 001 3±0. 000 9 ,P=0. 007）while in HCC-adjaeent tissues of hu man（0. 008 3±0. 006 4） it was significantly higher than that in normal liver tissues （P=0. 060）. There was no significant difference between HCC-adjacent tissues and HCC tissues （P= 0. 352）. The expression of Cyclin B1 in the HCC tissue significantly correlated with the clinical stage,the Portal Vein Tumor Thrombus,the recurre

关 键 词：细胞周期蛋白B1 跨种属 肝肿瘤 荧光定量PCR 印迹法 蛋白质 

分 类 号：R735.7[医药卫生—肿瘤]