四水合二氯化锰对大鼠移植性肝脏肿瘤的MR诊断研究  

Diagnostic study of manganese chloride tetrahydrate enhanced MRI in rat liver tumor

作　　者：姚楠[1] 黄德健[2] 高萌[1] 蒋翠花[1] 方志军[1] 汪俊瑛[1] 江骁[1] 张健[1] 倪以成[3] 

机构地区：[1]江苏省中医药研究院转化医学实验室,江苏南京210028 [2]江苏省中西医结合医院放射科 [3]比利时鲁汶大学医学院放射科

出　　处：《实用放射学杂志》2013年第10期1674-1677,1685,共5页Journal of Practical Radiology

基　　金：江苏省国际科技合作项目（BZ2010042）;国家自然科学基金项目（81071828）;江苏省自然科学基金项目（BK2010594）.

摘　　要：研究口服锰MR对比剂主要成分四水合二氯化锰对大鼠Walker-256移植性肝脏肿瘤的诊断效果,并与马根维显增强扫描结果进行比较。方法 制作大鼠Walker-256移植性肝脏肿瘤模型,肿瘤接种后第10天进行MR基线扫描,并将大鼠分为2组,一组灌胃四水合二氯化锰,4h后行肝脏T1WI序列扫描,另一组静脉注射马根维显后立即增强扫描,4h后再行T1WI序列扫描。比较2组增强扫描的肿瘤检出率和肝脏肿瘤对比率。扫描结束后处死大鼠,取出肝脏肿瘤进行病理学检查。结果 病理结果显示40个肿瘤病灶全部长出,四水合二氯化锰增强检出率为100%,马根维显立即增强检出率为95%,马根维显增强4h检出率为80%;四水合二氯化锰增强后肝脏肿瘤对比率由平扫时的0.14±0.05增加为0.35±0.07,马根维显组立即增强由平扫时的0.15±0.05增加为0.23±0.05,4h后下降至0.17±0.06,2组增强后对比率相比均有统计学意义（P〈0.01）。结论 四水合二氯化锰增强可以诊断肝脏转移瘤,肿瘤检出率与马根维显立即增强接近,肝脏肿瘤对比率高于马根维显。Objective To investigate the diagnostic value of manganese chloride tetrahydrate as an oral manganese based contrast agent in rat liver tumor model of Walker256 and to compare it with Gd-DTPA. Methods Walker-256 transplanted liver tumor mod- el was established 10 days after implantation of freshly harvested tumor pieces in liver lobes. Using a 1.5T MR set, sequences for all rats were acquired at baselines. The rats were divided into two groups. TIWI was obtained at 4 hours after the administration of manganese chloride tetrahydrate in group 1. Group 2 was examined with T2WI, T1WI＋contrast MR scanning. T1WI was performed again at 4 hours after intravenous injection of Gd-DTPA. The rate of detection of tumors and the livevto-tumor ratio of the two contrast media were compared. All rats were sacrificed after the MRI scanning and their tumors were dissected for histopathologic analysis. Results Forty tumors grew with success rate of 100% at 10 days after the implantation. Of 20 tumors, 20 were de- tected after oral ingestion of manganese chloride tetrahydrate at 4 hours （100%）; 19 were detected after intravenous Gd-DTPA （95%）, and 16 were detected at 4 hours （80%）. The mean liver-to-tumor ratio increased from0.14±0.05 before administration of contrast to 0.35±0.07 after manganese chloride tetrahydrate and from 0. 15±0.05 to 0.23±0.05 after Gd-DTPA. The liver to tumor SI ratio was significantly higher after manganese chloride tetrahydrate than after Gd-DTPA （P〈0. 01）. Conclusion MR scanning can be performed post-contrast after oral administration of manganese chloride tetrahydrate to detect liver tumor. Tumor detection rate of manganese chloride tetrahydrate was slightly higher than that of Gd-DTPA. The liver-to-tumor ratio of manganese chloride tetrahydrate was significantly higher than that of Gd-DTPA

关 键 词：四水合二氯化锰 口服锰对比剂 肝脏肿瘤 磁共振成像 鼠 

分 类 号：R735.7[医药卫生—肿瘤] R445.2[医药卫生—临床医学]