脑缺血损伤时脑片细胞内Ca^(2+)及脂质过氧化物的测定  

MEASUREMENT OF INTRACELLULAR Ca^(2+) AND LIPID PEROXIDE INBRAIN SLICES DURING CEREBRAL ISCHEMIA DAMAGE

作　　者：邢虹[1] 何其华[1] 袁兰[1] 丁亚男 徐家鸰[2] 吴本玠[2] 

机构地区：[1]北京医科大学医药分析中心,北京100083 [2]北京医科大学生物物理学系,北京100083

出　　处：《生物物理学报》2000年第4期813-818,共6页Acta Biophysica Sinica

基　　金："九五"国家医学科技攻关项目!96 -906 -02 -21

摘　　要：用插线法制作局灶性脑缺血/再灌损伤模型 ,激光共聚焦扫描显微镜观察活体脑片细胞内Ca2 +的分布及脂质过氧化物水平的动态变化 ,结果表明 :缺血1h时只有纹状体Ca2 +含量增加 ,而缺血4h时梗塞侧皮质、纹状体区域的Ca2 +和脂质过氧化物含量均明显增加 ;在有缺血损伤的各组再灌后细胞内Ca2 +与脂质过氧化物含量均进一步增高 ,且与缺血时间相关 ,而与再灌持续时间无明显关系 ;轻度缺血/再灌损伤时细胞内脂质过氧化物增加的发生较增钙反应为早 ;纹状体对缺血/再灌损伤比皮质敏感。The model of focal cerebral ischemia/reperfusion(I/R) was established in rats by reversibly inserting a nylon thread to observe dynamic changes of the intracellular Ca2+ and lipid peroxide in brain slices using laser confocal scanning microscope. The results showed: At ischemia 1 hour the Ca2+ was only increased in striatum,but at ischemia 4 hours the Ca2+ and lipid peroxide markedly increased in infarcted lateral of cortex and striatum; The Ca2+ and lipid peroxide were further increased at I/R, and being correlated to ischemia time, but not to reperfusion time; during slight I/R damage, the lipid peroxide increase appeared earlier than Ca2+;The striatum was more sensitive to ischemia/reperfusion than cortex is.

关 键 词：局灶性脑缺血 脂质过氧化物 钙离子 脑片细胞 

分 类 号：R743.31[医药卫生—神经病学与精神病学]