乙醛脱氢酶1在人直肠癌中的表达及其预后价值  

Expression of aldehyde dehydrogenase 1 in human rectal carcinoma tissues and its prognostic signiicance

作　　者：周飞[1,2] 穆寅东[3] 郁雷[4] 吴影[5] 张际绯[3] 

机构地区：[1]齐齐哈尔市中医医院肛肠外科,黑龙江齐齐哈尔161000 [2]黑龙江中医药大学,中西医结合专业黑龙江哈尔滨150001 [3]牡丹江医学院基础医学院组胚教研室,黑龙江牡丹江157000 [4]哈尔滨医科大学附属第二医院结直肠外科,黑龙江哈尔滨150001 [5]牡丹江医学院红旗医院病理科,黑龙江牡丹江157000

出　　处：《肿瘤》2013年第10期898-902,共5页Tumor

基　　金：黑龙江省自然科学基金资助项目(编号:D201259)

摘　　要：目的:探讨直肠癌组织中干细胞和肿瘤干细胞共同表达的标志物乙醛脱氢酶1(aldehyde dehydrogenase 1,ALDH1)的表达及其临床意义。方法:通过间接荧光抗体染色法检测50例直肠癌组织及其癌旁组织中ALDH1蛋白的表达,分析其与直肠癌患者临床病理参数之间的关系,Kaplan-Meier法分析直肠癌患者的5年生存率。结果:直肠癌组织中ALDH1蛋白的阳性表达率明显高于其癌旁组织(P<0.05)。ALDH1蛋白表达与肿瘤的分化程度、Duke’s分期和TNM分期有关(P<0.05)。ALDH1蛋白阳性表达的直肠癌患者5年生存率低于阴性表达的患者(P<0.05)。结论:直肠癌组织中ALDH1蛋白的表达与患者的不同临床病理参数和5年生存率有关。ObJective： To investigate the expression of CSC （cancer stem cell） and SC （stem cell） co- expressed marker ALDH1 （aldehyde dehydrogenase 1） in rectal carcinoma tissues and its clinical significance. Methods： The expressions of ALDH1 in rectal carcinoma tissues and the corresponding para-carcinoma tissues from 50 cases of rectal carcinoma were detected by indirect fluorescence antibody staining. The relationship between the expressions of ALDH1 and the clinicopathological features of patients with rectal carcinoma was analyzed. The five-year survival rate was estimated by Kaplan-Meier method. Results： The positive expression rate of ALDH1 in rectal carcinoma tissues was higher than that in the para-carcinoma tissues （P 〈 0.05）. The expression level of ALDH1 was associated with tumor differentiation, Duke＇s stage, and TNM stage （P 〈 0.05）. The five-year survival rate of patients with positive expression of ALDH1 was lower than that of patients without ALDH1 expression （P 〈 0.05）. Conclusion： The expression of ALDH1 is associated with certain clinicopathological features and the five-year survival rate of patients with rectal cancer.

关 键 词：直肠肿瘤 基因表达 干细胞 肿瘤干细胞 乙醛脱氢酶1 间接荧光抗体染色 

分 类 号：R735.37[医药卫生—肿瘤]