响应面优化产琥珀酸放线杆菌生产丁二酸  

Optimization of succinic acid fermentation with Actinobacillus succinogenes by response surface methodology

作　　者：申乃坤[1,2] 秦艳[2] 王青艳[2] 谢能中[2] 米慧芝[2] 朱绮霞[2] 廖思明[1,2] 黄日波[1,2] 

机构地区：[1]广西大学生命科学与技术学院广西亚热带生物资源保护利用重点实验室,广西南宁530005 [2]广西科学院非粮生物质酶解国家重点实验室国家非粮生物质能源工程技术研究中心广西生物炼制重点实验室,广西南宁530007

出　　处：《生物工程学报》2013年第10期1473-1483,共11页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.31160023);广西自然科学基金(No.2013GXNSFBA019102);广西科学院基金(No.13YJ22SW);八桂学者建设工程专项经费资助~~

摘　　要：丁二酸是一种重要的C4化合物平台,可以合成一系列重要化合物。文中对产琥珀酸放线杆菌Actinobacillus succinogenes GXAS137发酵生产丁二酸培养基成分进行优化。通过单因素和Plackett-Burman试验设计筛选出影响丁二酸发酵的重要参数,采用最陡爬坡实验逼近最大丁二酸生产区域后,利用Box-Behnken设计确定重要参数的最佳水平。筛选结果表明,影响丁二酸产量的重要参数是葡萄糖、酵母提取物和碱式碳酸镁浓度。最佳条件为(g/L):葡萄糖70.00,酵母提取物9.20,碱式碳酸镁58.10。优化后丁二酸产量达到47.64 g/L。与初始条件(36.89 g/L)相比,丁二酸浓度提高了29.14%。在最佳工艺条件下得到的试验结果与模型预测值很吻合,说明建立的模型是有效的。Succinic acid is an important C4 platform chemical in the synthesis of many commodity and special chemicals. In the present work, different compounds were evaluated for succinic acid production by Actinobacillus succinogenes GXAS137. Important parameters were screened by the single factor experiment and Plackeet-Burman design. Subsequently, the highest production of succinic acid was approached by the path of steepest ascent. Then, the optimum values of the parameters were obtained by Box-Behnken design. The results show that the important parameters were glucose, yeast extract and MgCO3 concentrations. The optimum condition was as follows （g/L）： glucose 70.00, yeast extract 9.20 and MgCO3 58.10. Succinic acid yield reached 47.64 g/L at the optimal condition. Succinic acid increased by 30% than that before the optimization （36.89 g/L）. Response surface methodology was proven to be a powerful tool to optimize succinic acid production.

关 键 词：产琥珀酸放线杆菌GXAS137 Plackett-Burman试验 响应面分析 丁二酸 

分 类 号：TQ921[轻工技术与工程—发酵工程]