人胃癌细胞原位移植裸鼠原发灶与肝转移灶体外化疗药敏性差异  

Difference in chemosensitivity in vitro between primary tumor and liver metastasis in nude mice with orthotopic implantation of human gastric cancer

作　　者：范立侨[1] 李勇[1] 赵群[1] 檀碧波[1] 孙川[1] 李兆星[1] 王冬[1] 刘羽[1] 

机构地区：[1]河北医科大学第四医院外三科,河北石家庄050011

出　　处：《中国普通外科杂志》2013年第10期1280-1284,共5页China Journal of General Surgery

基　　金：国家自然科学基金资助项目(81072033);河北省自然科学基金资助项目(C2010000619);河北省卫生厅科研基金资助项目(20110460);河北省普通高校强势特色学科资助项目(冀教高[2005]52)

摘　　要：目的:比较人胃癌原位移植裸鼠肝转移模型原发灶、肝转移灶肿瘤细胞对化疗药物敏感性。方法:将裸鼠皮下传代的SGC-7901细胞株实体瘤组织块移植于裸鼠胃壁,建立人胃癌裸鼠原位移植模型,待其发生肝转移后取胃原发灶、肝转移灶肿瘤细胞,SRB法检测肿瘤细胞对氟尿嘧啶(5-FU),顺铂(CDDP),奥沙利铂(L-OHP),表阿霉素(eADM),丝裂霉素(MMC),长春新碱(VCR),氨甲喋呤(MTX)7种化疗药物的体外敏感性。结果:成功建立裸鼠原位移植胃癌转移模型,肿瘤原位移植成瘤率100%,肝转移率75%;7种药物中L-OHP,VCR对原发灶肿瘤细胞的抑制率高于肝转移灶,而eADM,MMC对肝转移灶肿瘤细胞的抑制率高于原发灶(均P<0.05);5-FU,L-OHP,MTX对原发灶与肝转移灶的抑制率具正相关性(r=0.5203;0.4424;0.3851,均P<0.05)。结论:胃癌原位移植动物的原发灶和肝转移灶细胞的对化疗药物药敏性存在差异,以原发灶药敏检测结果指导针对肝转移灶的治疗可能是不准确的。Objective： To compare the sensitivity to chemotherapeutic agents between the tumor cells from primary and metastatic site in nude mice with orthotopic implantation of human gastric cancer. Methods： Orthotopic xenograft models of human gastric cancer were established in nude mice by intra-gastricwall inoculation of the fragments of tumor mass derived from SGC7901 cells which were subcutaneously grown in the donor mice. After metastases formation, the disaggregated tumor cells from primary site and liver metastases were collected, and the in vitro sensitivities of the cells to seven chemotherapy drugs that included fluorouracil （5-FU）, cisplatin （CDDP）, oxaliplatin （L-OHP）, epirubicin （eADM）, mitomycin （MMC）, vincristine （VCR） and methotrexate （MTX） were determined by sulforhodamine B （SRB） assay. Results： Orthopotic mouse models of stomach cancer were established successfully. The rate of primary tumor formation following orthopotic implantation was 100%, and the liver metastasis rate was 75%. Among the seven drugs, the inhibition rates of L- OHP and VCR for the tumor cells from primary site were higher than those from metastases, while the inhibition rates ofeADM and MMC for the tumor cells from metastases were higher than those from primary site （all P〈0.05）; the inhibition rates ofS-FU, L-OHP, MTX for the tumor cells between primary and metastatic sites had a positive correlation （r=0.5203, 0.4424 and 0.3851, all P〈0.05）. Conclusion： Chemotherapy sensitivity varies between the tumor cells from primary site and hepatic metastasis in nude mice with orthotopic implantation of stomach cancer, so the treatment for liver metastasis that is based on the drug sensitivity testing results from the primary tumor may not be accurate.

关 键 词：胃肿瘤 肿瘤移植 肿瘤转移 药物筛选试验 抗肿瘤 小鼠 裸 

分 类 号：R735.2[医药卫生—肿瘤]