炎症性肠病患者自噬相关IRGM基因的表达变化及临床意义  

The change of IRGM expression in IBD patients and its clinical significance

作　　者：陆忠凯[1] 陈志荣[1] 

机构地区：[1]南京医科大学附属苏州医院消化内科,江苏苏州215001

出　　处：《胃肠病学和肝病学杂志》2013年第10期1010-1012,共3页Chinese Journal of Gastroenterology and Hepatology

基　　金：江苏省苏州市科教兴卫青年基金(SWKQ1027)

摘　　要：目的探讨炎症性肠病患者自噬相关IRGM基因的表达变化及临床意义。方法收集2009年1月-2011年12月我院诊治的炎症性肠病患者共166例,其中溃疡性结肠炎患者98例,克隆恩病患者68例。收集患者的临床资料及其外周血5 ml,提取单个核细胞,采用RT-PCR法检测IRGM基因的表达变化,分析其表达变化的临床意义。50例健康人群作为对照组。结果对照组外周血IRGM仅少量表达(0.204±0.095),溃疡性结肠炎患者IRGM表达较对照组明显增高,且随着病情的加重IRGM表达量进行性增高(轻、中、重型分别为0.408±0.102、0.582±0.184和0.826±0.218),差异有统计学意义(P<0.05)。克隆恩病患者IRGM表达较对照组明显增高,且随着病情的加重IRGM表达量进行性增高(轻、中、重型分别为0.462±0.126、0.628±0.202和0.902±0.308),差异有统计学意义(P<0.05)。结论 IRGM基因可能参与炎症性肠病的起病,并与病情的严重程度相关。Objective To explore the expression and clinical significance of IRGM in IBD patients. Methods 166 cases of IBD patients including 98 cases of UC and 68 cases of CD from Jan. 2009 to Dee. 2011 were enrolled in this research. The peripheral blood was collected for mononuclear cell extraction. The expression of IRGM was detected by RT-PCR and its clinical significance was explored. Results There was little IRGM expression in control group （0. 204 ±0. 095）. And the IRGM expression was obviously elevated with the severity of UC. The IRGM expression in mild, moderate and severe subgroup of UC were 0. 408 ± 0. 102, 0. 582 ± 0. 184 and 0. 826 ± 0. 218, respectively （P 〈 0.05）. The IRGM expression in CD was much higher than that in the control group. And the IRGM expression was in- creased with severity of CD. The IRGM expression in mild, moderate and severe subgroup of CD were 0. 462 ± 0. 126, 0. 628 ± 0. 202 and 0. 902 ± 0. 308, respectively（P 〈 0.05）. Conclusion IRGM is possibly correlated with the patho- genesis and severity of IBD.

关 键 词：IRGM基因 炎症性肠病 自噬 

分 类 号：R574[医药卫生—消化系统]