CO_2气腹对人卵巢癌SKOV3细胞裸鼠移植瘤CXCL12、CXCR4表达的影响  

Effect of CO_2 pneumoperitoneum on the expression levels of CXCL12 and CXCR4 in nude mice xenografts from human ovarian cancer SKOV3 cells

作　　者：张颖[1,2] 刘莹[2] 符爱珍[2] 李元军[2] 范炳娟[2] 罗新[1] 

机构地区：[1]暨南大学附属第一医院,广州市510630 [2]广东医学院附属医院

出　　处：《中国肿瘤临床》2013年第20期1217-1220,共4页Chinese Journal of Clinical Oncology

基　　金：广东省科技计划项目(编号:2008B030301019)资助~~

摘　　要：目的:探讨二氧化碳(CO2)气腹在相同压力不同时间对卵巢癌裸鼠移植瘤CXCL12、CXCR4表达的影响。方法:建立40只人卵巢癌SKOV3细胞裸鼠移植瘤动物模型,随机分为4组,每组10只,分别为单纯麻醉组、开腹组、CO2气腹0.5 h组、CO2气腹1 h组,成瘤后按以上分组处理后,取出肿瘤组织并采用PCR法检测各组中CXCL12,CXCR4 mRNA的表达,Western blot法检测CXCL12,CXCR4蛋白表达。结果:40只人卵巢癌SKOV3细胞裸鼠移植瘤动物模型建立成功,经实验处理后RT-PCR提示CO2气腹1 h组瘤体CXCL12 mRNA表达显著高于其余3组,而CXCR4 mRNA无表达差异,Western blot也提示CO2气腹1 h组瘤体CX CL12蛋白表达显著高于其余3组,而CXCR4蛋白无表达差异。结论:CO2气腹1 h组可提高卵巢癌SKOV3细胞CXCL12的表达水平,可能对腹腔移植瘤生长具有促进作用,而对转移影响意义不大。Objective： To investigate the effect of the expression levels of CXCL12 and CXCR4 of carbon dioxide （CO2） pneu- moperitoneum on nude mice xenografts from human ovarian cancer SKOV3 cells in the same pressure at different times. Methods： A total of 40 animal models of human ovarian cancer SKOV3 cell-bearing nude mice xenografts were established. The animals were ran- domly divided into four groups （n=10）, namely, the simple anesthesia group, 0.5 h laparotomy group, and 0.5 and 1 h CO2 pneumoperi- toneum groups. After the aforementioned treatments in the groups, the expression levels of CXCL12 and CXCR4 mRNAs of tumor tis- sues were detected by reverse transcription-polymerase chain reaction （RT-PCR）, whereas those of the CXCL12 and CXCR4 proteins were detected by Western blot. Results： The 40 animal models of human ovarian cancer SKOV3 cell-bearing nude mice were success- fully established. After the treatment, RT-PCR results reveal that the CXCL12 mRNA expression was significantly higher in the 1 h CO2 pneumoperitoneum group than that in the other three groups. However, no differences were observed in the CXCR4 mRNA expression of each group. Western blot analysis also obtained the same results. Conclusion： The expression level of CXCL12 in SKOV3 cells of the 1 h COs pneumoperitoneum group increased, which possibly promoted the growth of peritoneal tumors, and had a slight effect on tu- mor metastasis

关 键 词：气腹 卵巢癌 CXCL12 CXCR4 

分 类 号：R737.31[医药卫生—肿瘤]