miR-146a对新生隐球菌诱生THP-1细胞分泌炎症因子的调节作用  

Regulatory effects of miR-146a on Cryptococcus neoformans-induced inflammation factors production in THP-1 cells

作　　者：韩志君[1] 黄红宇[1] 张敏[1] 魏云玉[1] 刘晶波[1] 严子禾[1] 

机构地区：[1]无锡市第二人民医院检验科,214002

出　　处：《中华微生物学和免疫学杂志》2013年第10期740-743,共4页Chinese Journal of Microbiology and Immunology

基　　金：无锡市科技支撑计划

摘　　要：目的：探讨miR-146a对新生隐球菌诱导 THP-1细胞分泌IL-1β和TNF-α的调控作用。方法采用加热法灭活新生隐球菌，荧光定量 RT-PCR 及 ELISA 检测灭活新生隐球菌经miR-146a促进人THP-1细胞分泌IL-1β和TNF-α的作用，同时了解THP-1细胞Dectin-1和TLR4封闭前后IL-1β和TNF-α水平变化。构建miR-146a mimics和inhibitor转染的THP-1细胞株，观察灭活新生隐球菌诱导不同细胞株分泌IL-1β和TNF-α水平差异。结果灭活新生隐球菌可以上调THP-1细胞miR-146a表达水平，封闭 Dectin -1和 TLR4可使 miR-146a 表达水平下调。 miR-146a mimics 或inhibitor转染后可分别抑制和促进灭活新生隐球菌诱导的THP-1细胞分泌IL-1β和TNF-α能力。结论新生隐球菌可经Dectin-1和TLR4上调THP-1细胞miR-146a表达水平，miR-146a具有负调控新生隐球菌诱导THP-1细胞分泌IL-1β和TNF-α的作用。Objective To investigate the regulatory effects of miR-146a on inflammation factors production in C ryptococcus neoformans treated THP-1 cells.Methods Cryptcoo ccus neoformans strains were heat killed .Fluorescence quantitative RTP-CR and ELISA were used to detect the levels of IL -1βand TNF-αin THP-1 cells treated with heat-killed Cryptococcus neoformans.In addition, the production of IL-1βand TNF-αwere analyzed before and after Dectin-1or TLR 4 blocked .THP-1 cell lines that were respectively transfected with miR-146 a mimics and inhibitors were constructed and the production of IL-1βand TNF-αin these cell lines were analyzed after interfered with Cryptococcus neoformans.Results With the interference of heat-killed Cryptococcus neoformans, the expression of miR-146a was up-regulated in THP-1 cells, but was down-regulated after Dectin-1 or TLR4 blocked.The expressions of IL-1βand TNF-αinduced by heat-killed Cryptococcus neoformans were enhanced in miR-146a mimics transfected THP-1 cells, but was inhibi-ted in inhibitors transfected THP-1 cells.Conclusion Heat-killed Cryptococcus neoformans could up-regu-late miR-146a expression in THP-1 cells via Dectin-1 and TLR.miR-146a could negatively regulate the ex-pressions of IL-1βand TNF-αinduced by Cryptococcus neoformans.

关 键 词：新生隐球菌 MIR-146A 免疫调节 

分 类 号：R363[医药卫生—病理学]