芝麻NBS类抗茎点枯病相关基因的克隆与分析  

Cloning and Analysis of NBS-type Resistance Genes in Sesame against Macrophomina phaseolina

作　　者：于沐[1] 刘红彦[1,2] 苗红梅[3] 田保明[1] 

机构地区：[1]郑州大学生物工程系,河南郑州450001 [2]河南省农业科学院植物保护研究所,河南郑州450002 [3]河南省农业科学院芝麻研究中心,河南郑州450002

出　　处：《河南农业科学》2013年第10期74-78,98,共6页Journal of Henan Agricultural Sciences

基　　金：现代农业产业技术体系建设专项资金资助项目(CARS-15-1-05);河南省创新型科技人才队伍建设工程

摘　　要：根据前期获得的芝麻抗茎点枯病NBS-RGAs序列与河南省芝麻研究中心提供的EST序列比对结果,设计4对特异引物,从10个对茎点枯病表现不同抗性的芝麻品种中扩增得到16条RGAs(抗病基因同源序列),GenBank登录号分别为KC477692～KC477707,它们编码的氨基酸序列均具有NBS(核苷酸结合位点)类型抗病基因的特征结构域。Blastx分析结果表明,16个RGAs基因编码的部分氨基酸序列与已知NBS类抗病蛋白同源性为35%～52%。聚类分析发现这些RGAs基因聚为4类,且均为non TIR-NBS类型,为进一步筛选芝麻抗茎点枯病基因奠定了基础。In this study,four pairs of specific primers were designed based on comparison of NBS- RGAs of stem blight-resistant cultivars with：：： sesame EST sequences, with which sixteen full- length RGA genes （GenBank accession number： KC477692--KC477707） were cloned from ten different resistance cultivars against Macrophomina phaseolina. All of these RGA gene sequences contained the characteristic domains of NBS-type disease resistance genes, and shared the amino acid identities of 35 %-52% with NBS-type disease resistance protein according to Blastx analyses. They were divided into four types by MEGA 4.0,and all belonged to non TIR-NBS type. The result gives the foundation for further screening sesame resistance genes against Macrophomina phaseolina.

关 键 词：芝麻 茎点枯病 核苷酸结合位点 抗病基因同源序列 序列分析 

分 类 号：S435.653[农业科学—农业昆虫与害虫防治]