响应面法优化酵母培养物的制备工艺  

Optimization of production conditions for yeast culture by response surface methodology

作　　者：张连忠[1] 

机构地区：[1]唐山师范学院生命科学系,河北唐山063000

出　　处：《广东农业科学》2013年第20期108-110,共3页Guangdong Agricultural Sciences

基　　金：河北省教育厅项目(Z2009467);唐山市科学技术研究与发展计划项目(09140202A-1)

摘　　要：以秸秆、淀粉、豆粕、酒糟等农副产品为基质,制备酵母培养物微生态饲料,研究酵母培养物的最佳制备工艺条件。采用响应面Box-Benhken中心组合设计法,考察反应体系中接种量、含水量、温度和时间4个因素及其相互作用对酵母培养物中粗蛋白含量的影响。获得优化后的酵母培养物最佳制备条件为:接种量0.11%,含水量48.0%,培养温度28.6℃,培养时间50.3 h。经过发酵酵母培养物的粗蛋白含量可达32.91%。Straw, starch, soybean meal and distillers＇grains were sellected as raw material to prepare yeast culture microecological feed. The optimal technology conditions were studied. This research studied the effects of inoculum size, moisture content, temperature, time and their interaction on the content of crude protein in the yeast culture by response surface methodology. The optimal production conditions for yeast culture were as follows, inoculation amount was 0.11%, moisture content was 48.0%, culture temperature was 28.6℃ and fermentation time was 50.3 h. The crude protein content of yeast culture by fermentation was 32.91% .

关 键 词：酵母培养物 响应面法 生产工艺 

分 类 号：Q815[生物学—生物工程]